1 | <?xml version="1.0" ?> |
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2 | <?xml-stylesheet type="text/xsl" href="raw-data-types.xsl"?> |
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3 | <!DOCTYPE raw-data-types SYSTEM "raw-data-types.dtd" > |
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4 | <!-- |
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5 | $Id: raw-data-types.xml 2959 2006-11-27 11:36:37Z nicklas $ |
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6 | |
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7 | Copyright (C) 2005-2006 Jari Häkkinen, Nicklas Nordborg |
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8 | |
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9 | This file is part of BASE - BioArray Software Environment. |
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10 | Available at http://base.thep.lu.se/ |
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11 | |
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12 | BASE is free software; you can redistribute it and/or modify it |
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13 | under the terms of the GNU General Public License as published by |
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14 | the Free Software Foundation; either version 2 of the License, or |
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15 | (at your option) any later version. |
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16 | |
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17 | BASE is distributed in the hope that it will be useful, but |
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18 | WITHOUT ANY WARRANTY; without even the implied warranty of |
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19 | MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU |
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20 | General Public License for more details. |
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21 | |
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22 | You should have received a copy of the GNU General Public License |
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23 | along with this program; if not, write to the Free Software |
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24 | Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA |
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25 | 02111-1307, USA. |
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26 | --> |
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27 | <raw-data-types> |
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28 | |
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29 | <raw-data-type |
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30 | id="genepix" |
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31 | channels="2" |
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32 | name="GenePix" |
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33 | table="RawDataGenePix" |
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34 | > |
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35 | <property |
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36 | name="diameter" |
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37 | title="Spot diameter" |
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38 | description="The diameter of the spot in pixels" |
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39 | column="diameter" |
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40 | type="float" |
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41 | /> |
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42 | <property |
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43 | name="ch1FgMedian" |
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44 | title="Channel 1 foreground median" |
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45 | description="The median of the foreground intensity in channel 1" |
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46 | column="ch1_fg_median" |
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47 | type="float" |
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48 | channel="1" |
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49 | /> |
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50 | <property |
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51 | name="ch1FgMean" |
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52 | title="Channel 1 foreground mean" |
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53 | description="The mean of the foreground intensity in channel 1" |
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54 | column="ch1_fg_mean" |
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55 | type="float" |
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56 | channel="1" |
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57 | /> |
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58 | <property |
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59 | name="ch1FgSd" |
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60 | title="Channel 1 foreground standard deviation" |
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61 | description="The standard deviation of the foreground intensity in channel 1" |
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62 | column="ch1_fg_sd" |
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63 | type="float" |
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64 | channel="1" |
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65 | /> |
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66 | <property |
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67 | name="ch1BgMedian" |
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68 | title="Channel 1 background median" |
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69 | description="The median of the background intensity in channel 1" |
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70 | column="ch1_bg_median" |
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71 | type="float" |
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72 | channel="1" |
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73 | /> |
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74 | <property |
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75 | name="ch1BgMean" |
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76 | title="Channel 1 background mean" |
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77 | description="The mean of the background intensity in channel 1" |
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78 | column="ch1_bg_mean" |
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79 | type="float" |
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80 | channel="1" |
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81 | /> |
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82 | <property |
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83 | name="ch1BgSd" |
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84 | title="Channel 1 background standard deviation" |
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85 | description="The standard deviation of the background intensity in channel 1" |
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86 | column="ch1_bg_sd" |
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87 | type="float" |
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88 | channel="1" |
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89 | /> |
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90 | <property |
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91 | name="ch1PercSd1" |
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92 | title="Percent pixels within 1 standard deviation" |
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93 | description="Percentage of pixels within 1 standard deviation in channel 1" |
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94 | column="ch1_perc_sd1" |
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95 | type="int" |
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96 | channel="1" |
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97 | /> |
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98 | <property |
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99 | name="ch1PercSd2" |
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100 | title="Percent pixels within 2 standard deviations" |
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101 | description="Percentage of pixels within 2 standard deviations in channel 1" |
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102 | column="ch1_perc_sd2" |
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103 | type="int" |
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104 | channel="1" |
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105 | /> |
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106 | <property |
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107 | name="ch1PercSat" |
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108 | title="Percent saturated pixels" |
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109 | description="Percentage of saturated pixels in channel 1" |
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110 | column="ch1_perc_sat" |
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111 | type="int" |
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112 | channel="1" |
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113 | /> |
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114 | |
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115 | <property |
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116 | name="ch2FgMedian" |
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117 | title="Channel 2 foreground median" |
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118 | description="The median of the foreground intensity in channel 2" |
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119 | column="ch2_fg_median" |
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120 | type="float" |
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121 | channel="2" |
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122 | /> |
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123 | <property |
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124 | name="ch2FgMean" |
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125 | title="Channel 2 foreground mean" |
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126 | description="The mean of the foreground intensity in channel 2" |
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127 | column="ch2_fg_mean" |
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128 | type="float" |
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129 | channel="2" |
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130 | /> |
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131 | <property |
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132 | name="ch2FgSd" |
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133 | title="Channel 2 foreground standard deviation" |
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134 | description="The standard deviation of the foreground intensity in channel 2" |
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135 | column="ch2_fg_sd" |
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136 | type="float" |
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137 | channel="2" |
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138 | /> |
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139 | <property |
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140 | name="ch2BgMedian" |
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141 | title="Channel 2 background median" |
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142 | description="The median of the background intensity in channel 2" |
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143 | column="ch2_bg_median" |
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144 | type="float" |
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145 | channel="2" |
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146 | /> |
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147 | <property |
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148 | name="ch2BgMean" |
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149 | title="Channel 2 background mean" |
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150 | description="The mean of the background intensity in channel 2" |
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151 | column="ch2_bg_mean" |
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152 | type="float" |
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153 | channel="2" |
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154 | /> |
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155 | <property |
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156 | name="ch2BgSd" |
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157 | title="Channel 2 background standard deviation" |
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158 | description="The standard deviation of the background intensity in channel 2" |
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159 | column="ch2_bg_sd" |
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160 | type="float" |
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161 | channel="2" |
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162 | /> |
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163 | <property |
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164 | name="ch2PercSd1" |
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165 | title="Percent pixels within 1 standard deviation" |
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166 | description="Percentage of pixels within 1 standard deviation in channel 2" |
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167 | column="ch2_perc_sd1" |
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168 | type="int" |
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169 | channel="2" |
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170 | /> |
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171 | <property |
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172 | name="ch2PercSd2" |
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173 | title="Percent pixels within 2 standard deviations" |
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174 | description="Percentage of pixels within 2 standard deviations in channel 2" |
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175 | column="ch2_perc_sd2" |
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176 | type="int" |
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177 | channel="2" |
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178 | /> |
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179 | <property |
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180 | name="ch2PercSat" |
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181 | title="Percent saturated pixels" |
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182 | description="Percentage of saturated pixels in channel 2" |
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183 | column="ch2_perc_sat" |
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184 | type="int" |
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185 | channel="2" |
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186 | /> |
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187 | |
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188 | <property |
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189 | name="ratiosSd" |
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190 | title="Standard deviation of ratios" |
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191 | description="Standard deviation of the intensity ratios for all pixels" |
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192 | column="ratios_sd" |
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193 | type="float" |
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194 | /> |
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195 | <property |
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196 | name="rgnRatio" |
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197 | title="Rgn ratio" |
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198 | description="Rgn ratio" |
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199 | column="rgn_ratio" |
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200 | type="float" |
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201 | /> |
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202 | <property |
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203 | name="rgnR2" |
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204 | title="Rgn R2" |
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205 | description="Rgn R2" |
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206 | column="rgn_r2" |
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207 | type="float" |
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208 | /> |
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209 | |
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210 | <property |
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211 | name="fgPixels" |
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212 | title="Foreground pixels" |
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213 | description="Number of foreground pixels" |
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214 | column="fg_pixels" |
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215 | type="int" |
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216 | /> |
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217 | <property |
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218 | name="bgPixels" |
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219 | title="Background pixels" |
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220 | description="Number of background pixels" |
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221 | column="bg_pixels" |
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222 | type="int" |
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223 | /> |
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224 | <property |
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225 | name="flags" |
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226 | title="Flags" |
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227 | description="Flags" |
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228 | column="flags" |
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229 | type="int" |
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230 | /> |
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231 | <property |
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232 | name="mValue" |
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233 | title="M value" |
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234 | description="M value" |
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235 | column="m_value" |
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236 | type="float" |
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237 | /> |
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238 | <property |
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239 | name="CV" |
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240 | title="CV" |
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241 | description="CV" |
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242 | column="cv" |
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243 | type="float" |
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244 | /> |
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245 | <intensity-formula |
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246 | name="mean" |
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247 | title="Mean FG - Mean BG" |
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248 | description="Subtract mean background from mean foreground" |
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249 | > |
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250 | <formula |
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251 | channel="1" |
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252 | expression="raw('ch1FgMean') - raw('ch1BgMean')" |
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253 | /> |
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254 | <formula |
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255 | channel="2" |
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256 | expression="raw('ch2FgMean') - raw('ch2BgMean')" |
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257 | /> |
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258 | </intensity-formula> |
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259 | <intensity-formula |
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260 | name="median" |
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261 | title="Median FG - Median BG" |
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262 | description="Subtract meadian background from median foreground" |
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263 | > |
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264 | <formula |
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265 | channel="1" |
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266 | expression="raw('ch1FgMedian') - raw('ch1BgMedian')" |
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267 | /> |
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268 | <formula |
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269 | channel="2" |
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270 | expression="raw('ch2FgMedian') - raw('ch2BgMedian')" |
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271 | /> |
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272 | </intensity-formula> |
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273 | <intensity-formula |
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274 | name="medianmean" |
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275 | title="Median FG - Mean BG" |
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276 | description="Subtract mean background from median foreground" |
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277 | > |
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278 | <formula |
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279 | channel="1" |
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280 | expression="raw('ch1FgMedian') - raw('ch1BgMean')" |
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281 | /> |
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282 | <formula |
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283 | channel="2" |
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284 | expression="raw('ch2FgMedian') - raw('ch2BgMean')" |
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285 | /> |
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286 | </intensity-formula> |
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287 | <intensity-formula |
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288 | name="meanmedian" |
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289 | title="Mean FG - Median BG" |
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290 | description="Subtract median background from mean foreground" |
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291 | > |
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292 | <formula |
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293 | channel="1" |
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294 | expression="raw('ch1FgMean') - raw('ch1BgMedian')" |
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295 | /> |
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296 | <formula |
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297 | channel="2" |
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298 | expression="raw('ch2FgMean') - raw('ch2BgMedian')" |
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299 | /> |
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300 | </intensity-formula> |
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301 | <intensity-formula |
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302 | name="meanfg" |
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303 | title="Mean FG" |
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304 | description="Mean foreground" |
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305 | > |
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306 | <formula |
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307 | channel="1" |
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308 | expression="raw('ch1FgMean')" |
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309 | /> |
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310 | <formula |
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311 | channel="2" |
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312 | expression="raw('ch2FgMean')" |
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313 | /> |
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314 | </intensity-formula> |
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315 | <intensity-formula |
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316 | name="medianfg" |
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317 | title="Median FG" |
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318 | description="Median foreground" |
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319 | > |
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320 | <formula |
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321 | channel="1" |
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322 | expression="raw('ch1FgMedian')" |
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323 | /> |
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324 | <formula |
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325 | channel="2" |
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326 | expression="raw('ch2FgMedian')" |
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327 | /> |
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328 | </intensity-formula> |
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329 | <intensity-formula |
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330 | name="medianfgmeanmedianbg" |
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331 | title="Median FG - Mean(Median BG)" |
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332 | description="Subtract mean of median backgrounds from median foreground" |
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333 | > |
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334 | <formula |
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335 | channel="1" |
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336 | expression="raw('ch1FgMedian') - mean('ch1BgMedian')" |
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337 | /> |
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338 | <formula |
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339 | channel="2" |
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340 | expression="raw('ch2FgMedian') - mean('ch2BgMedian')" |
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341 | /> |
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342 | </intensity-formula> |
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343 | <intensity-formula |
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344 | name="meanfgmeanmeanbg" |
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345 | title="Mean FG - Mean(Mean BG)" |
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346 | description="Subtract mean of mean backgrounds from mean foreground" |
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347 | > |
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348 | <formula |
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349 | channel="1" |
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350 | expression="raw('ch1FgMean') - mean('ch1BgMean')" |
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351 | /> |
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352 | <formula |
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353 | channel="2" |
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354 | expression="raw('ch2FgMean') - mean('ch2BgMean')" |
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355 | /> |
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356 | </intensity-formula> |
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357 | <intensity-formula |
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358 | name="medianfgmeanmeanbg" |
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359 | title="Median FG - Mean(Mean BG)" |
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360 | description="Subtract mean of mean backgrounds from median foreground" |
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361 | > |
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362 | <formula |
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363 | channel="1" |
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364 | expression="raw('ch1FgMedian') - mean('ch1BgMean')" |
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365 | /> |
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366 | <formula |
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367 | channel="2" |
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368 | expression="raw('ch2FgMedian') - mean('ch2BgMean')" |
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369 | /> |
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370 | </intensity-formula> |
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371 | <intensity-formula |
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372 | name="meanfgmeanmedianbg" |
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373 | title="Mean FG - Mean(Median BG)" |
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374 | description="Subtract mean of median backgrounds from mean foreground" |
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375 | > |
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376 | <formula |
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377 | channel="1" |
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378 | expression="raw('ch1FgMean') - mean('ch1BgMedian')" |
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379 | /> |
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380 | <formula |
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381 | channel="2" |
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382 | expression="raw('ch2FgMean') - mean('ch2BgMedian')" |
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383 | /> |
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384 | </intensity-formula> |
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385 | </raw-data-type> |
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386 | |
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387 | <raw-data-type |
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388 | id="bzscan" |
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389 | channels="1" |
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390 | name="BZScan" |
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391 | table="RawDataBZScan" |
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392 | > |
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393 | <property |
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394 | name="QImageConst" |
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395 | title="Q(image,const)" |
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396 | description="Spot intensity based on user-provided diameter and measured data." |
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397 | column="q_image_const" |
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398 | type="float" |
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399 | /> |
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400 | <property |
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401 | name="QImageVar" |
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402 | title="Q(image,var)" |
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403 | description="Spot intensity based on computed diameter and measured data." |
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404 | column="q_image_var" |
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405 | type="float" |
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406 | /> |
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407 | <property |
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408 | name="QFitConst" |
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409 | title="Q(fit,const)" |
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410 | description="Spot intensity based on user-provided diameter and theoretical fit to the data." |
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411 | column="q_fit_const" |
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412 | type="float" |
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413 | /> |
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414 | <property |
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415 | name="QFitVar" |
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416 | title="Q(fit,var)" |
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417 | description="Spot intensity based on computed diameter and and theoretical fit to the data." |
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418 | column="q_fit_var" |
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419 | type="float" |
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420 | /> |
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421 | <property |
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422 | name="FitCorrection" |
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423 | title="Fit correction" |
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424 | description="Background level by unit surface." |
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425 | column="fit_correction" |
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426 | type="float" |
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427 | /> |
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428 | <property |
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429 | name="QM" |
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430 | title="Quality metric" |
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431 | description="Quality metric: distance between measured data and theoretical fit." |
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432 | column="quality_metric" |
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433 | type="float" |
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434 | /> |
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435 | <property |
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436 | name="SpotQuality" |
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437 | title="Spot quality" |
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438 | description="Present/Absent (automatic guess)." |
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439 | column="spot_quality" |
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440 | type="boolean" |
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441 | /> |
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442 | <property |
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443 | name="OvershiningCorrection" |
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444 | title="Overshining correction" |
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445 | description="Correction for overshining by unit radius." |
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446 | column="overshining" |
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447 | type="float" |
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448 | /> |
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449 | <intensity-formula |
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450 | name="QImageConst" |
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451 | title="Q(image,const)" |
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452 | description="Spot intensity based on user-provided diameter and measured data" |
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453 | > |
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454 | <formula |
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455 | channel="1" |
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456 | expression="raw('QImageConst')" |
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457 | /> |
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458 | </intensity-formula> |
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459 | <intensity-formula |
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460 | name="QImageVar" |
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461 | title="Q(image,var)" |
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462 | description="Spot intensity based on computed diameter and measured data" |
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463 | > |
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464 | <formula |
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465 | channel="1" |
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466 | expression="raw('QImageVar')" |
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467 | /> |
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468 | </intensity-formula> |
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469 | <intensity-formula |
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470 | name="QFitConst" |
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471 | title="Q(fit,const)" |
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472 | description="Spot intensity based on user-provided diameter and theoretical fit to the data" |
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473 | > |
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474 | <formula |
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475 | channel="1" |
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476 | expression="raw('QFitConst')" |
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477 | /> |
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478 | </intensity-formula> |
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479 | <intensity-formula |
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480 | name="QFitVar" |
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481 | title="Q(fit,var)" |
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482 | description="Spot intensity based on computed diameter and and theoretical fit to the data" |
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483 | > |
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484 | <formula |
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485 | channel="1" |
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486 | expression="raw('QFitVar')" |
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487 | /> |
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488 | </intensity-formula> |
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489 | </raw-data-type> |
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490 | |
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491 | <raw-data-type |
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492 | id="aida" |
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493 | channels="2" |
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494 | name="AIDA" |
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495 | table="RawDataAida" |
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496 | > |
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497 | <property |
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498 | name="area" |
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499 | title="Area" |
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500 | description="Spot area in pixels." |
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501 | column="area" |
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502 | type="float" |
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503 | /> |
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504 | <property |
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505 | name="ch1Integral" |
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506 | title="Channel 1 integral" |
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507 | description="Integrated raw intensity of spot channel 1." |
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508 | column="ch1_integral" |
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509 | type="float" |
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510 | channel="1" |
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511 | /> |
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512 | <property |
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513 | name="ch1Mean" |
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514 | title="Channel 1 mean" |
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515 | description="Mean (Integral/Area) channel 1." |
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516 | column="ch1_mean" |
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517 | type="float" |
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518 | channel="1" |
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519 | /> |
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520 | <property |
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521 | name="ch1Median" |
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522 | title="Channel 1 median" |
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523 | description="Median of the raw pixel intensity of spot channel 1." |
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524 | column="ch1_median" |
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525 | type="float" |
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526 | channel="1" |
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527 | /> |
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528 | <property |
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529 | name="ch1Bkg" |
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530 | title="Channel 1 background" |
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531 | description="Standard deviation of background channel 1." |
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532 | column="ch1_bkg" |
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533 | type="float" |
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534 | channel="1" |
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535 | /> |
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536 | <property |
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537 | name="ch1Sat" |
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538 | title="Channel 1 saturated" |
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539 | description="Measure of saturation pixels channel 1." |
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540 | column="ch1_sat" |
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541 | type="float" |
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542 | channel="1" |
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543 | /> |
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544 | <property |
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545 | name="ch1Hom" |
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546 | title="Channel 1 homogenity" |
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547 | description="Percentage of dot deviation from homogenous intensity distribution channel 1." |
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548 | column="ch1_hom" |
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549 | type="float" |
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550 | channel="1" |
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551 | /> |
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552 | <property |
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553 | name="ch1Quality" |
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554 | title="Channel 1 quality" |
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555 | description="Quality flag channel 1." |
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556 | column="ch1_quality" |
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557 | type="boolean" |
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558 | channel="1" |
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559 | /> |
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560 | <property |
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561 | name="ch2Integral" |
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562 | title="Channel 2 integral" |
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563 | description="Integrated raw intensity of spot channel 2." |
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564 | column="ch2_integral" |
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565 | type="float" |
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566 | channel="2" |
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567 | /> |
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568 | <property |
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569 | name="ch2Mean" |
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570 | title="Channel 2 mean" |
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571 | description="Mean (Integral/Area) channel 2." |
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572 | column="ch2_mean" |
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573 | type="float" |
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574 | channel="2" |
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575 | /> |
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576 | <property |
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577 | name="ch2Median" |
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578 | title="Channel 2 median" |
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579 | description="Median of the raw pixel intensity of spot channel 2." |
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580 | column="ch2_median" |
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581 | type="float" |
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582 | channel="2" |
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583 | /> |
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584 | <property |
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585 | name="ch2Bkg" |
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586 | title="Channel 2 background" |
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587 | description="Standard deviation of background channel 2." |
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588 | column="ch2_bkg" |
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589 | type="float" |
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590 | channel="2" |
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591 | /> |
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592 | <property |
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593 | name="ch2Sat" |
---|
594 | title="Channel 2 saturated" |
---|
595 | description="Measure of saturation pixels channel 2." |
---|
596 | column="ch2_sat" |
---|
597 | type="float" |
---|
598 | channel="2" |
---|
599 | /> |
---|
600 | <property |
---|
601 | name="ch2Hom" |
---|
602 | title="Channel 2 homogenity" |
---|
603 | description="Percentage of dot deviation from homogenous intensity distribution channel 2." |
---|
604 | column="ch2_hom" |
---|
605 | type="float" |
---|
606 | channel="2" |
---|
607 | /> |
---|
608 | <property |
---|
609 | name="ch2Quality" |
---|
610 | title="Channel 2 quality" |
---|
611 | description="Quality flag channel 2." |
---|
612 | column="ch2_quality" |
---|
613 | type="boolean" |
---|
614 | channel="2" |
---|
615 | /> |
---|
616 | <intensity-formula |
---|
617 | name="mean" |
---|
618 | title="Mean" |
---|
619 | description="Mean of the raw pixel intensity (Integral/Area)" |
---|
620 | > |
---|
621 | <formula |
---|
622 | channel="1" |
---|
623 | expression="raw('ch1Mean')" |
---|
624 | /> |
---|
625 | <formula |
---|
626 | channel="2" |
---|
627 | expression="raw('ch2Mean')" |
---|
628 | /> |
---|
629 | </intensity-formula> |
---|
630 | <intensity-formula |
---|
631 | name="median" |
---|
632 | title="Median" |
---|
633 | description="Median of the raw pixel intensity" |
---|
634 | > |
---|
635 | <formula |
---|
636 | channel="1" |
---|
637 | expression="raw('ch1Median')" |
---|
638 | /> |
---|
639 | <formula |
---|
640 | channel="2" |
---|
641 | expression="raw('ch2Median')" |
---|
642 | /> |
---|
643 | </intensity-formula> |
---|
644 | </raw-data-type> |
---|
645 | |
---|
646 | <raw-data-type |
---|
647 | id="affymetrix" |
---|
648 | channels="1" |
---|
649 | name="Affymetrix" |
---|
650 | storage="file" |
---|
651 | > |
---|
652 | </raw-data-type> |
---|
653 | |
---|
654 | <raw-data-type |
---|
655 | id="agilent" |
---|
656 | channels="2" |
---|
657 | name="Agilent" |
---|
658 | table="RawDataAgilent" |
---|
659 | > |
---|
660 | <property |
---|
661 | name="LogRatio" |
---|
662 | title="Log ratio" |
---|
663 | description="log(REDsignal/GREENsignal) per feature. Signals in both channels are post dye normalization. If SURROGATES are disabled, then this value will be set to: -2 if DyeNormRedSig <= 0.0 amp; DyeNormGreenSig > 0.0; 2 if DyeNormRedSig > 0.0 & DyeNormGreenSig <= 0.0; and 0 if DyeNormRedSig <= 0.0 amp; DyeNormGreenSig <= 0.0." |
---|
664 | column="log_ratio" |
---|
665 | type="float" |
---|
666 | /> |
---|
667 | <property |
---|
668 | name="PValueLogRatio" |
---|
669 | title="P value log ratio" |
---|
670 | description="Significance level of the Log Ratio computed for a feature." |
---|
671 | column="pvalue_log_ratio" |
---|
672 | type="float" |
---|
673 | /> |
---|
674 | <property |
---|
675 | name="gSurrogateUsed" |
---|
676 | title="G surrogate used" |
---|
677 | description="The green surrogate value used." |
---|
678 | column="g_surrogate_used" |
---|
679 | channel="1" |
---|
680 | type="float" |
---|
681 | /> |
---|
682 | <property |
---|
683 | name="rSurrogateUsed" |
---|
684 | title="R surrogate used" |
---|
685 | description="The red surrogate value used." |
---|
686 | column="r_surrogate_used" |
---|
687 | channel="2" |
---|
688 | type="float" |
---|
689 | /> |
---|
690 | <property |
---|
691 | name="gIsFound" |
---|
692 | title="G is found" |
---|
693 | description="A boolean used to flag found (strong) features. The flag is applied independently in each channel. A feature is considered found if the found spot centroid is within the bounds of the spot deviation limit with respect to corresponding nominal centroid. NOTE: IsFound was previously termed IsStrong." |
---|
694 | column="g_is_found" |
---|
695 | channel="1" |
---|
696 | type="boolean" |
---|
697 | /> |
---|
698 | <property |
---|
699 | name="rIsFound" |
---|
700 | title="R is found" |
---|
701 | description="A boolean used to flag found (strong) features. The flag is applied independently in each channel. A feature is considered found if the found spot centroid is within the bounds of the spot deviation limit with respect to corresponding nominal centroid. NOTE: IsFound was previously termed IsStrong." |
---|
702 | column="r_is_found" |
---|
703 | channel="2" |
---|
704 | type="boolean" |
---|
705 | /> |
---|
706 | <property |
---|
707 | name="gProcessedSignal" |
---|
708 | title="G processed signal" |
---|
709 | description="The propagated feature signal, per channel, used for computation of log ratio." |
---|
710 | column="g_processed_signal" |
---|
711 | channel="1" |
---|
712 | type="float" |
---|
713 | /> |
---|
714 | <property |
---|
715 | name="rProcessedSignal" |
---|
716 | title="R processed signal" |
---|
717 | description="The propagated feature signal, per channel, used for computation of log ratio." |
---|
718 | column="r_processed_signal" |
---|
719 | channel="2" |
---|
720 | type="float" |
---|
721 | /> |
---|
722 | <property |
---|
723 | name="gNumPixOLHi" |
---|
724 | title="G high outlier pixels" |
---|
725 | description="Number of outlier pixels per feature with intensity > upper threshold set via the pixel outlier rejection method. The number is computed independently in each channel. These pixels are omitted from all subsequent calculations." |
---|
726 | column="g_high_outlier_pixels" |
---|
727 | channel="1" |
---|
728 | type="int" |
---|
729 | /> |
---|
730 | <property |
---|
731 | name="rNumPixOLHi" |
---|
732 | title="R high outlier pixels" |
---|
733 | description="Number of outlier pixels per feature with intensity > upper threshold set via the pixel outlier rejection method. The number is computed independently in each channel. These pixels are omitted from all subsequent calculations." |
---|
734 | column="r_high_outlier_pixels" |
---|
735 | channel="2" |
---|
736 | type="int" |
---|
737 | /> |
---|
738 | <property |
---|
739 | name="gNumPixOLLo" |
---|
740 | title="G low outlier pixels" |
---|
741 | description="Number of outlier pixels per feature with intensity < lower threshold set via the pixel outlier rejection method. The number is computed independently in each channel. NOTE: The pixel outlier method is the ONLY step that removes data in Feature Extraction." |
---|
742 | column="g_low_outlier_pixels" |
---|
743 | channel="1" |
---|
744 | type="int" |
---|
745 | /> |
---|
746 | <property |
---|
747 | name="rNumPixOLLo" |
---|
748 | title="R low outlier pixels" |
---|
749 | description="Number of outlier pixels per feature with intensity < lower threshold set via the pixel outlier rejection method. The number is computed independently in each channel. NOTE: The pixel outlier method is the ONLY step that removes data in Feature Extraction." |
---|
750 | column="r_low_outlier_pixels" |
---|
751 | channel="2" |
---|
752 | type="int" |
---|
753 | /> |
---|
754 | <property |
---|
755 | name="gNumPix" |
---|
756 | title="G pixels" |
---|
757 | description="Total number of pixels used to compute feature statistics; ie. total number of inlier pixels/per spot, computed independently in each channel. The number of inlier pixels are the same in both channels." |
---|
758 | column="g_pixels" |
---|
759 | channel="1" |
---|
760 | type="int" |
---|
761 | /> |
---|
762 | <property |
---|
763 | name="rNumPix" |
---|
764 | title="R pixels" |
---|
765 | description="Total number of pixels used to compute feature statistics; ie. total number of inlier pixels/per spot, computed independently in each channel. The number of inlier pixels are the same in both channels." |
---|
766 | column="r_pixels" |
---|
767 | channel="2" |
---|
768 | type="int" |
---|
769 | /> |
---|
770 | <property |
---|
771 | name="gMeanSignal" |
---|
772 | title="G mean signal" |
---|
773 | description="Raw mean signal of feature in green channel." |
---|
774 | column="g_mean_signal" |
---|
775 | channel="1" |
---|
776 | type="float" |
---|
777 | /> |
---|
778 | <property |
---|
779 | name="rMeanSignal" |
---|
780 | title="R mean signal" |
---|
781 | description="Raw mean signal of feature in red channel." |
---|
782 | column="r_mean_signal" |
---|
783 | channel="2" |
---|
784 | type="float" |
---|
785 | /> |
---|
786 | <property |
---|
787 | name="gMedianSignal" |
---|
788 | title="G median signal" |
---|
789 | description="Raw median signal of feature in green channel." |
---|
790 | column="g_median_signal" |
---|
791 | channel="1" |
---|
792 | type="float" |
---|
793 | /> |
---|
794 | <property |
---|
795 | name="rMedianSignal" |
---|
796 | title="R median signal" |
---|
797 | description="Raw median signal of feature in red channel." |
---|
798 | column="r_median_signal" |
---|
799 | channel="2" |
---|
800 | type="float" |
---|
801 | /> |
---|
802 | <property |
---|
803 | name="gBGNumPix" |
---|
804 | title="G background pixels" |
---|
805 | description="Total number of pixels used to compute Local BG statistics per spot; ie. Total number of BG inlier pixels. This number is computed independently in each channel." |
---|
806 | column="g_bg_pixels" |
---|
807 | channel="1" |
---|
808 | type="int" |
---|
809 | /> |
---|
810 | <property |
---|
811 | name="rBGNumPix" |
---|
812 | title="R background pixels" |
---|
813 | description="Total number of pixels used to compute Local BG statistics per spot; ie. Total number of BG inlier pixels. This number is computed independently in each channel." |
---|
814 | column="r_bg_pixels" |
---|
815 | channel="2" |
---|
816 | type="int" |
---|
817 | /> |
---|
818 | <property |
---|
819 | name="gBGMeanSignal" |
---|
820 | title="G mean background" |
---|
821 | description="Mean local background signal (local to corresponding feature) computed per channel." |
---|
822 | column="g_bg_mean_signal" |
---|
823 | channel="1" |
---|
824 | type="float" |
---|
825 | /> |
---|
826 | <property |
---|
827 | name="rBGMeanSignal" |
---|
828 | title="R mean background" |
---|
829 | description="Mean local background signal (local to corresponding feature) computed per channel." |
---|
830 | column="r_bg_mean_signal" |
---|
831 | channel="2" |
---|
832 | type="float" |
---|
833 | /> |
---|
834 | <property |
---|
835 | name="gBGMedianSignal" |
---|
836 | title="G median background" |
---|
837 | description="Median local background signal (local to corresponding feature) computed per channel." |
---|
838 | column="g_bg_median_signal" |
---|
839 | channel="1" |
---|
840 | type="float" |
---|
841 | /> |
---|
842 | <property |
---|
843 | name="rBGMedianSignal" |
---|
844 | title="R median background" |
---|
845 | description="Median local background signal (local to corresponding feature) computed per channel." |
---|
846 | column="r_bg_median_signal" |
---|
847 | channel="2" |
---|
848 | type="float" |
---|
849 | /> |
---|
850 | <property |
---|
851 | name="gNumSatPix" |
---|
852 | title="G saturated pixels" |
---|
853 | description="Total number of saturated pixels per feature, computed per channel." |
---|
854 | column="g_sat_pixels" |
---|
855 | channel="1" |
---|
856 | type="int" |
---|
857 | /> |
---|
858 | <property |
---|
859 | name="rNumSatPix" |
---|
860 | title="R saturated pixels" |
---|
861 | description="Total number of saturated pixels per feature, computed per channel." |
---|
862 | column="r_sat_pixels" |
---|
863 | channel="2" |
---|
864 | type="int" |
---|
865 | /> |
---|
866 | <property |
---|
867 | name="gIsSaturated" |
---|
868 | title="G is saturated" |
---|
869 | description="Boolean flag indicating if a feature is saturated or not. A feature is saturated if 50% of the pixels in a feature are above the saturation threshold. A value of 1 means saturated." |
---|
870 | column="g_is_saturated" |
---|
871 | channel="1" |
---|
872 | type="boolean" |
---|
873 | /> |
---|
874 | <property |
---|
875 | name="rIsSaturated" |
---|
876 | title="R is saturated" |
---|
877 | description="Boolean flag indicating if a feature is saturated or not. A feature is saturated if 50% of the pixels in a feature are above the saturation threshold. A value of 1 means saturated." |
---|
878 | column="r_is_saturated" |
---|
879 | channel="2" |
---|
880 | type="boolean" |
---|
881 | /> |
---|
882 | <property |
---|
883 | name="PixCorrelation" |
---|
884 | title="Pix correlation" |
---|
885 | description="Ratio of estimated feature covariance in RedGreen space to product of feature Standard Deviation in Red Green space. The covariance of two features measures their tendency to vary together, i.e. to co-vary. In this case, it is a cumulative quantitation of the tendency of pixels belonging to a particular feature in Red and Green spaces to co-vary." |
---|
886 | column="pix_correlation" |
---|
887 | type="float" |
---|
888 | /> |
---|
889 | <property |
---|
890 | name="BGPixCorrelation" |
---|
891 | title="BG pix correlation" |
---|
892 | description="Ratio of estimated feature covariance in RedGreen space to product of feature Standard Deviation in Red Green space. The covariance of two features measures their tendency to vary together, i.e. to co-vary. In this case, it is a cumulative quantitation of the tendency of pixels belonging to a particular feature in Red and Green spaces to co-vary." |
---|
893 | column="bg_pix_correlation" |
---|
894 | type="float" |
---|
895 | /> |
---|
896 | <property |
---|
897 | name="gIsFeatNonUnifOL" |
---|
898 | title="G non-uniform outlier" |
---|
899 | description="Boolean flag indicating if a feature is a NonUniformity Outlier or not. A feature is non-uniform if the pixel noise of feature exceeds a threshold established for a 'uniform' feature. A 1 indicates Feature is a non-uniformity outlier." |
---|
900 | column="g_nonuniform_outlier" |
---|
901 | channel="1" |
---|
902 | type="boolean" |
---|
903 | /> |
---|
904 | <property |
---|
905 | name="rIsFeatNonUnifOL" |
---|
906 | title="R non-uniform outlier" |
---|
907 | description="Boolean flag indicating if a feature is a NonUniformity Outlier or not. A feature is non-uniform if the pixel noise of feature exceeds a threshold established for a 'uniform' feature. A 1 indicates Feature is a non-uniformity outlier." |
---|
908 | column="r_nonuniform_outlier" |
---|
909 | channel="2" |
---|
910 | type="boolean" |
---|
911 | /> |
---|
912 | <property |
---|
913 | name="gIsBGNonUnifOL" |
---|
914 | title="G bg non-uniform outlier" |
---|
915 | description="Boolean flag indicating if a background is a NonUniformity Outlier or not. A feature is non-uniform if the pixel noise of feature exceeds a threshold established for a 'uniform' feature. A 1 indicates Feature is a non-uniformity outlier." |
---|
916 | column="g_bg_nonuniform_outlier" |
---|
917 | channel="1" |
---|
918 | type="boolean" |
---|
919 | /> |
---|
920 | <property |
---|
921 | name="rIsBGNonUnifOL" |
---|
922 | title="R bg non-uniform outlier" |
---|
923 | description="Boolean flag indicating if a background is a NonUniformity Outlier or not. A feature is non-uniform if the pixel noise of feature exceeds a threshold established for a 'uniform' feature. A 1 indicates Feature is a non-uniformity outlier." |
---|
924 | column="r_bg_nonuniform_outlier" |
---|
925 | channel="2" |
---|
926 | type="boolean" |
---|
927 | /> |
---|
928 | <property |
---|
929 | name="gIsFeatPopnOL" |
---|
930 | title="G population outlier" |
---|
931 | description="Boolean flag indicating if a feature is a Population Outlier or not. Probes with replicate features on a microarray are examined using population statistics. A feature is a population outlier if its signal is less than a lower threshold or exceeds an upper threshold determined using the interquartile range (i.e. IQR) of the population. A 1 indicates Feature is a population outlier." |
---|
932 | column="g_population_outlier" |
---|
933 | channel="1" |
---|
934 | type="boolean" |
---|
935 | /> |
---|
936 | <property |
---|
937 | name="rIsFeatPopnOL" |
---|
938 | title="R population outlier" |
---|
939 | description="Boolean flag indicating if a feature is a Population Outlier or not. Probes with replicate features on a microarray are examined using population statistics. A feature is a population outlier if its signal is less than a lower threshold or exceeds an upper threshold determined using the interquartile range (i.e. IQR) of the population. A 1 indicates Feature is a population outlier." |
---|
940 | column="r_population_outlier" |
---|
941 | channel="2" |
---|
942 | type="boolean" |
---|
943 | /> |
---|
944 | <property |
---|
945 | name="gIsBGPopnOL" |
---|
946 | title="G bg population outlier" |
---|
947 | description="Boolean flag indicating if a background is a Population Outlier or not. Probes with replicate features on a microarray are examined using population statistics. A feature is a population outlier if its signal is less than a lower threshold or exceeds an upper threshold determined using the interquartile range (i.e. IQR) of the population. A 1 indicates background is a population outlier." |
---|
948 | column="g_bg_population_outlier" |
---|
949 | channel="1" |
---|
950 | type="boolean" |
---|
951 | /> |
---|
952 | <property |
---|
953 | name="rIsBGPopnOL" |
---|
954 | title="R bg population outlier" |
---|
955 | description="Boolean flag indicating if a background is a Population Outlier or not. Probes with replicate features on a microarray are examined using population statistics. A feature is a population outlier if its signal is less than a lower threshold or exceeds an upper threshold determined using the interquartile range (i.e. IQR) of the population. A 1 indicates background is a population outlier." |
---|
956 | column="r_bg_population_outlier" |
---|
957 | channel="2" |
---|
958 | type="boolean" |
---|
959 | /> |
---|
960 | <property |
---|
961 | name="IsManualFlag" |
---|
962 | title="Manual flag" |
---|
963 | description="If a feature has been moved manually this flag will be set to 1." |
---|
964 | column="manual_flag" |
---|
965 | type="boolean" |
---|
966 | /> |
---|
967 | <property |
---|
968 | name="gBGSubSignal" |
---|
969 | title="G net signal" |
---|
970 | description="The net green signal following the subtraction of the background from the raw mean green signal." |
---|
971 | column="g_net_signal" |
---|
972 | channel="1" |
---|
973 | type="float" |
---|
974 | /> |
---|
975 | <property |
---|
976 | name="rBGSubSignal" |
---|
977 | title="R net signal" |
---|
978 | description="The net red signal following the subtraction of the background from the raw mean red signal." |
---|
979 | column="r_net_signal" |
---|
980 | channel="2" |
---|
981 | type="float" |
---|
982 | /> |
---|
983 | <property |
---|
984 | name="BGSubSigCorrelation" |
---|
985 | title="BG correlation" |
---|
986 | description="Ratio of estimated background subtracted feature signal covariance in RG space to product of background subtracted feature Standard Deviation in RG space." |
---|
987 | column="bg_correlation" |
---|
988 | type="float" |
---|
989 | /> |
---|
990 | <property |
---|
991 | name="gIsPosAndSignif" |
---|
992 | title="G > 0 and significant" |
---|
993 | description="Boolean flag indicating if the mean signal of a feature is greater than the corresponding background and if this difference is significant. Significance is established via a 2-sided t-test against the user-settable maximum p-value. A 1 indicates Feature is positive and significant above background." |
---|
994 | column="g_significant" |
---|
995 | channel="1" |
---|
996 | type="boolean" |
---|
997 | /> |
---|
998 | <property |
---|
999 | name="rIsPosAndSignif" |
---|
1000 | title="R > 0 and significant" |
---|
1001 | description="Boolean flag indicating if the mean signal of a feature is greater than the corresponding background and if this difference is significant. Significance is established via a 2-sided t-test against the user-settable maximum p-value. A 1 indicates Feature is positive and significant above background." |
---|
1002 | column="r_significant" |
---|
1003 | channel="2" |
---|
1004 | type="boolean" |
---|
1005 | /> |
---|
1006 | <property |
---|
1007 | name="gPValFeatEqBG" |
---|
1008 | title="G p-value" |
---|
1009 | description="P-value from t-test of significance between green Mean signal and green background." |
---|
1010 | column="g_pvalue" |
---|
1011 | channel="1" |
---|
1012 | type="float" |
---|
1013 | /> |
---|
1014 | <property |
---|
1015 | name="rPValFeatEqBG" |
---|
1016 | title="R p-value" |
---|
1017 | description="P-value from t-test of significance between red Mean signal and red background." |
---|
1018 | column="r_pvalue" |
---|
1019 | channel="2" |
---|
1020 | type="float" |
---|
1021 | /> |
---|
1022 | <property |
---|
1023 | name="gNumBGUsed" |
---|
1024 | title="G background pixels" |
---|
1025 | description="A count of the number of background pixels used in the green channel." |
---|
1026 | column="g_numbg_used" |
---|
1027 | channel="1" |
---|
1028 | type="int" |
---|
1029 | /> |
---|
1030 | <property |
---|
1031 | name="rNumBGUsed" |
---|
1032 | title="R background pixels" |
---|
1033 | description="A count of the number of background pixels used in the red channel." |
---|
1034 | column="r_numbg_used" |
---|
1035 | channel="2" |
---|
1036 | type="int" |
---|
1037 | /> |
---|
1038 | <property |
---|
1039 | name="gIsWellAboveBG" |
---|
1040 | title="G well above bg" |
---|
1041 | description="Boolean flag indicating if a feature is well above background or not. A feature must be gIsPosAndSignif and additionally the gBGSubSignal must be greater than 2.6*g(r)BG_SD. A 1 indicates a feature IsWellAboveBG." |
---|
1042 | column="g_above_bg" |
---|
1043 | channel="1" |
---|
1044 | type="boolean" |
---|
1045 | /> |
---|
1046 | <property |
---|
1047 | name="rIsWellAboveBG" |
---|
1048 | title="R well above bg" |
---|
1049 | description="Boolean flag indicating if a feature is well above background or not. A feature must be gIsPosAndSignif and additionally the gBGSubSignal must be greater than 2.6*g(r)BG_SD. A 1 indicates a feature IsWellAboveBG." |
---|
1050 | column="r_above_bg" |
---|
1051 | channel="2" |
---|
1052 | type="boolean" |
---|
1053 | /> |
---|
1054 | <property |
---|
1055 | name="gBGUsed" |
---|
1056 | title="G bg used" |
---|
1057 | description="Background value subtracted from the raw mean signal to generate the BG subtracted signal; this value is computed per channel. If global BG subtraction is used the column is identical for every feature in a given channel." |
---|
1058 | column="g_bg_used" |
---|
1059 | channel="1" |
---|
1060 | type="float" |
---|
1061 | /> |
---|
1062 | <property |
---|
1063 | name="rBGUsed" |
---|
1064 | title="R bg used" |
---|
1065 | description="Background value subtracted from the raw mean signal to generate the BG subtracted signal; this value is computed per channel. If global BG subtraction is used the column is identical for every feature in a given channel." |
---|
1066 | column="r_bg_used" |
---|
1067 | channel="2" |
---|
1068 | type="float" |
---|
1069 | /> |
---|
1070 | <property |
---|
1071 | name="IsNormalization" |
---|
1072 | title="Normalization" |
---|
1073 | description="A boolean flag which indicates if a feature is used to measure dye bias. A 1 incidates the value was used." |
---|
1074 | column="is_normalization" |
---|
1075 | type="boolean" |
---|
1076 | /> |
---|
1077 | <property |
---|
1078 | name="gDyeNormSignal" |
---|
1079 | title="G normalized signal" |
---|
1080 | description="The dye normalized signal in the green channel." |
---|
1081 | column="g_normalized" |
---|
1082 | channel="1" |
---|
1083 | type="float" |
---|
1084 | /> |
---|
1085 | <property |
---|
1086 | name="rDyeNormSignal" |
---|
1087 | title="R normalized signal" |
---|
1088 | description="The dye normalized signal in the green channel." |
---|
1089 | column="r_normalized" |
---|
1090 | channel="2" |
---|
1091 | type="float" |
---|
1092 | /> |
---|
1093 | <property |
---|
1094 | name="DyeNormCorrelation" |
---|
1095 | title="Normalized correlation" |
---|
1096 | description="Dye normalized red and green pixel correlation." |
---|
1097 | column="norm_correlation" |
---|
1098 | type="float" |
---|
1099 | /> |
---|
1100 | <property |
---|
1101 | name="ErrorModel" |
---|
1102 | title="Error model" |
---|
1103 | description="ErrorModel Indicates the error model that the user chose for feature extraction. A 0 indicates use of the Propogated Error Model. A 1 indicates use of the Universal Error Model." |
---|
1104 | column="error_model" |
---|
1105 | type="int" |
---|
1106 | /> |
---|
1107 | <property |
---|
1108 | name="xDev" |
---|
1109 | title="X dev" |
---|
1110 | description="Used to compute the Log Ratio PValue." |
---|
1111 | column="x_dev" |
---|
1112 | type="float" |
---|
1113 | /> |
---|
1114 | <property |
---|
1115 | name="gSpatialDetrendIsInFilteredSet" |
---|
1116 | title="G spatial trend" |
---|
1117 | description="Set to true for a given feature if it is part of the filtered set used to fit the surface measuring the spatial trend across the microarray. This feature is considered part of the locally weighted x% of the features." |
---|
1118 | column="g_spatial_trend" |
---|
1119 | channel="1" |
---|
1120 | type="boolean" |
---|
1121 | /> |
---|
1122 | <property |
---|
1123 | name="rSpatialDetrendIsInFilteredSet" |
---|
1124 | title="R spatial trend" |
---|
1125 | description="Set to true for a given feature if it is part of the filtered set used to fit the surface measuring the spatial trend across the microarray. This feature is considered part of the locally weighted x% of the features." |
---|
1126 | column="r_spatial_trend" |
---|
1127 | channel="2" |
---|
1128 | type="boolean" |
---|
1129 | /> |
---|
1130 | <property |
---|
1131 | name="gSpatialDetrendSurfaceValue" |
---|
1132 | title="G surface value" |
---|
1133 | description="Value of the smoothed surface derived from the set of features that are part of SpatialDetrendIsInFilteredSet." |
---|
1134 | column="g_surface_value" |
---|
1135 | channel="1" |
---|
1136 | type="float" |
---|
1137 | /> |
---|
1138 | <property |
---|
1139 | name="rSpatialDetrendSurfaceValue" |
---|
1140 | title="R surface value" |
---|
1141 | description="Value of the smoothed surface derived from the set of features that are part of SpatialDetrendIsInFilteredSet." |
---|
1142 | column="r_surface_value" |
---|
1143 | channel="2" |
---|
1144 | type="float" |
---|
1145 | /> |
---|
1146 | <intensity-formula |
---|
1147 | name="mean" |
---|
1148 | title="Mean FG - Mean BG" |
---|
1149 | description="Subtract mean background from mean foreground" |
---|
1150 | > |
---|
1151 | <formula |
---|
1152 | channel="1" |
---|
1153 | expression="raw('gMeanSignal') - raw('gBGMeanSignal')" |
---|
1154 | /> |
---|
1155 | <formula |
---|
1156 | channel="2" |
---|
1157 | expression="raw('rMeanSignal') - raw('rBGMeanSignal')" |
---|
1158 | /> |
---|
1159 | </intensity-formula> |
---|
1160 | <intensity-formula |
---|
1161 | name="median" |
---|
1162 | title="Median FG - Median BG" |
---|
1163 | description="Subtract meadian background from median foreground" |
---|
1164 | > |
---|
1165 | <formula |
---|
1166 | channel="1" |
---|
1167 | expression="raw('gMedianSignal') - raw('gBGMedianSignal')" |
---|
1168 | /> |
---|
1169 | <formula |
---|
1170 | channel="2" |
---|
1171 | expression="raw('rMedianSignal') - raw('rBGMedianSignal')" |
---|
1172 | /> |
---|
1173 | </intensity-formula> |
---|
1174 | <intensity-formula |
---|
1175 | name="medianmean" |
---|
1176 | title="Median FG - Mean BG" |
---|
1177 | description="Subtract mean background from median foreground" |
---|
1178 | > |
---|
1179 | <formula |
---|
1180 | channel="1" |
---|
1181 | expression="raw('gMedianSignal') - raw('gBGMeanSignal')" |
---|
1182 | /> |
---|
1183 | <formula |
---|
1184 | channel="2" |
---|
1185 | expression="raw('rMedianSignal') - raw('rBGMeanSignal')" |
---|
1186 | /> |
---|
1187 | </intensity-formula> |
---|
1188 | <intensity-formula |
---|
1189 | name="meanmedian" |
---|
1190 | title="Mean FG - Median BG" |
---|
1191 | description="Subtract median background from mean foreground" |
---|
1192 | > |
---|
1193 | <formula |
---|
1194 | channel="1" |
---|
1195 | expression="raw('gMeanSignal') - raw('gBGMedianSignal')" |
---|
1196 | /> |
---|
1197 | <formula |
---|
1198 | channel="2" |
---|
1199 | expression="raw('rMeanSignal') - raw('rBGMedianSignal')" |
---|
1200 | /> |
---|
1201 | </intensity-formula> |
---|
1202 | <intensity-formula |
---|
1203 | name="meanfg" |
---|
1204 | title="Mean FG" |
---|
1205 | description="Mean foreground" |
---|
1206 | > |
---|
1207 | <formula |
---|
1208 | channel="1" |
---|
1209 | expression="raw('gMeanSignal')" |
---|
1210 | /> |
---|
1211 | <formula |
---|
1212 | channel="2" |
---|
1213 | expression="raw('rMeanSignal')" |
---|
1214 | /> |
---|
1215 | </intensity-formula> |
---|
1216 | <intensity-formula |
---|
1217 | name="medianfg" |
---|
1218 | title="Median FG" |
---|
1219 | description="Median foreground" |
---|
1220 | > |
---|
1221 | <formula |
---|
1222 | channel="1" |
---|
1223 | expression="raw('gMedianSignal')" |
---|
1224 | /> |
---|
1225 | <formula |
---|
1226 | channel="2" |
---|
1227 | expression="raw('rMedianSignal')" |
---|
1228 | /> |
---|
1229 | </intensity-formula> |
---|
1230 | <intensity-formula |
---|
1231 | name="medianfgmeanmedianbg" |
---|
1232 | title="Median FG - Mean(Median BG)" |
---|
1233 | description="Subtract mean of median backgrounds from median foreground" |
---|
1234 | > |
---|
1235 | <formula |
---|
1236 | channel="1" |
---|
1237 | expression="raw('gMedianSignal') - mean('gBGMedianSignal')" |
---|
1238 | /> |
---|
1239 | <formula |
---|
1240 | channel="2" |
---|
1241 | expression="raw('rMedianSignal') - mean('rBGMedianSignal')" |
---|
1242 | /> |
---|
1243 | </intensity-formula> |
---|
1244 | <intensity-formula |
---|
1245 | name="meanfgmeanmeanbg" |
---|
1246 | title="Mean FG - Mean(Mean BG)" |
---|
1247 | description="Subtract mean of mean backgrounds from mean foreground" |
---|
1248 | > |
---|
1249 | <formula |
---|
1250 | channel="1" |
---|
1251 | expression="raw('gMeanSignal') - mean('gBGMeanSignal')" |
---|
1252 | /> |
---|
1253 | <formula |
---|
1254 | channel="2" |
---|
1255 | expression="raw('rMeanSignal') - mean('rBGMeanSignal')" |
---|
1256 | /> |
---|
1257 | </intensity-formula> |
---|
1258 | <intensity-formula |
---|
1259 | name="medianfgmeanmeanbg" |
---|
1260 | title="Median FG - Mean(Mean BG)" |
---|
1261 | description="Subtract mean of mean backgrounds from median foreground" |
---|
1262 | > |
---|
1263 | <formula |
---|
1264 | channel="1" |
---|
1265 | expression="raw('gMedianSignal') - mean('gBGMeanSignal')" |
---|
1266 | /> |
---|
1267 | <formula |
---|
1268 | channel="2" |
---|
1269 | expression="raw('rMedianSignal') - mean('rBGMeanSignal')" |
---|
1270 | /> |
---|
1271 | </intensity-formula> |
---|
1272 | <intensity-formula |
---|
1273 | name="meanfgmeanmedianbg" |
---|
1274 | title="Mean FG - Mean(Median BG)" |
---|
1275 | description="Subtract mean of median backgrounds from mean foreground" |
---|
1276 | > |
---|
1277 | <formula |
---|
1278 | channel="1" |
---|
1279 | expression="raw('gMeanSignal') - mean('gBGMedianSignal')" |
---|
1280 | /> |
---|
1281 | <formula |
---|
1282 | channel="2" |
---|
1283 | expression="raw('rMeanSignal') - mean('rBGMedianSignal')" |
---|
1284 | /> |
---|
1285 | </intensity-formula> |
---|
1286 | </raw-data-type> |
---|
1287 | |
---|
1288 | <raw-data-type |
---|
1289 | id="chipskipper" |
---|
1290 | channels="2" |
---|
1291 | name="ChipSkipper" |
---|
1292 | table="RawDataChipSkipper" |
---|
1293 | > |
---|
1294 | <property |
---|
1295 | name="Line_Counter" |
---|
1296 | title="Line counter" |
---|
1297 | description="Line counter." |
---|
1298 | column="line_counter" |
---|
1299 | type="int" |
---|
1300 | /> |
---|
1301 | <property |
---|
1302 | name="Flags" |
---|
1303 | title="Flags" |
---|
1304 | description="Quality Flags: SNR1,SNR2 = low signal quality in color channel Cy5, Cy3; GS = low geometric score; MB = manually flagged bad." |
---|
1305 | column="flags" |
---|
1306 | type="string" |
---|
1307 | /> |
---|
1308 | <property |
---|
1309 | name="GS_Flags" |
---|
1310 | title="GS flags" |
---|
1311 | description="P = pass or M = marginal flag." |
---|
1312 | column="gs_flags" |
---|
1313 | type="string" |
---|
1314 | /> |
---|
1315 | <property |
---|
1316 | name="Cy3_Volume" |
---|
1317 | title="Cy3 volume" |
---|
1318 | description="Total integrated signal intensity in color channel Cy3." |
---|
1319 | column="cy3_volume" |
---|
1320 | channel="1" |
---|
1321 | type="int" |
---|
1322 | /> |
---|
1323 | <property |
---|
1324 | name="Cy3_AvVolpixel" |
---|
1325 | title="Cy3 average intensity" |
---|
1326 | description="Average intensity per pixel in color channel Cy3." |
---|
1327 | column="cy3_avg" |
---|
1328 | channel="1" |
---|
1329 | type="float" |
---|
1330 | /> |
---|
1331 | <property |
---|
1332 | name="Cy3_BGArith" |
---|
1333 | title="Cy3 background (arithmetic)" |
---|
1334 | description="Arithmetic average for local background in color channel Cy3." |
---|
1335 | column="cy3_bg_arith" |
---|
1336 | channel="1" |
---|
1337 | type="float" |
---|
1338 | /> |
---|
1339 | <property |
---|
1340 | name="Cy3_BGGeom" |
---|
1341 | title="Cy3 background (geometric)" |
---|
1342 | description="Geometric average for local background in color channel Cy3." |
---|
1343 | column="cy3_bg_geom" |
---|
1344 | channel="1" |
---|
1345 | type="float" |
---|
1346 | /> |
---|
1347 | <property |
---|
1348 | name="Cy3_BGMedian" |
---|
1349 | title="Cy3 background (median)" |
---|
1350 | description="Median average for local background in color channel Cy3." |
---|
1351 | column="cy3_bg_median" |
---|
1352 | channel="1" |
---|
1353 | type="float" |
---|
1354 | /> |
---|
1355 | <property |
---|
1356 | name="Cy3_BG_Tot" |
---|
1357 | title="Cy3 background (total)" |
---|
1358 | description="Total integrated background in color channel Cy3." |
---|
1359 | column="cy3_bg_total" |
---|
1360 | channel="1" |
---|
1361 | type="float" |
---|
1362 | /> |
---|
1363 | <property |
---|
1364 | name="Cy3_VolBG" |
---|
1365 | title="Cy3 reduced intensity" |
---|
1366 | description="Background reduced intensity of colour channel Cy3." |
---|
1367 | column="cy3_reduced" |
---|
1368 | channel="1" |
---|
1369 | type="float" |
---|
1370 | /> |
---|
1371 | <property |
---|
1372 | name="Cy3_Spotarea" |
---|
1373 | title="Cy3 spot area" |
---|
1374 | description="Area of spot in colour channel Cy3 in pixels." |
---|
1375 | column="cy3_area" |
---|
1376 | channel="1" |
---|
1377 | type="float" |
---|
1378 | /> |
---|
1379 | <property |
---|
1380 | name="Cy3_Satpixel" |
---|
1381 | title="Cy3 saturated pixels" |
---|
1382 | description="Number of saturated pixel in color channel Cy3." |
---|
1383 | column="cy3_saturated" |
---|
1384 | channel="1" |
---|
1385 | type="float" |
---|
1386 | /> |
---|
1387 | <property |
---|
1388 | name="Cy5_Volume" |
---|
1389 | title="Cy5 volume" |
---|
1390 | description="Total integrated signal intensity in color channel Cy5." |
---|
1391 | column="cy5_volume" |
---|
1392 | channel="2" |
---|
1393 | type="int" |
---|
1394 | /> |
---|
1395 | <property |
---|
1396 | name="Cy5_AvVolpixel" |
---|
1397 | title="Cy5 average intensity" |
---|
1398 | description="Average intensity per pixel in color channel Cy5." |
---|
1399 | column="cy5_avg" |
---|
1400 | channel="2" |
---|
1401 | type="float" |
---|
1402 | /> |
---|
1403 | <property |
---|
1404 | name="Cy5_BGArith" |
---|
1405 | title="Cy5 background (arithmetic)" |
---|
1406 | description="Arithmetic average for local background in color channel Cy5." |
---|
1407 | column="cy5_bg_arith" |
---|
1408 | channel="2" |
---|
1409 | type="float" |
---|
1410 | /> |
---|
1411 | <property |
---|
1412 | name="Cy5_BGGeom" |
---|
1413 | title="Cy5 background (geometric)" |
---|
1414 | description="Geometric average for local background in color channel Cy5." |
---|
1415 | column="cy5_bg_geom" |
---|
1416 | channel="2" |
---|
1417 | type="float" |
---|
1418 | /> |
---|
1419 | <property |
---|
1420 | name="Cy5_BGMedian" |
---|
1421 | title="Cy5 background (median)" |
---|
1422 | description="Median average for local background in color channel Cy5." |
---|
1423 | column="cy5_bg_median" |
---|
1424 | channel="2" |
---|
1425 | type="float" |
---|
1426 | /> |
---|
1427 | <property |
---|
1428 | name="Cy5_BG_Tot" |
---|
1429 | title="Cy5 background (total)" |
---|
1430 | description="Total integrated background in color channel Cy5." |
---|
1431 | column="cy5_bg_total" |
---|
1432 | channel="2" |
---|
1433 | type="float" |
---|
1434 | /> |
---|
1435 | <property |
---|
1436 | name="Cy5_VolBG" |
---|
1437 | title="Cy5 reduced intensity" |
---|
1438 | description="Background reduced intensity of colour channel Cy5." |
---|
1439 | column="cy5_reduced" |
---|
1440 | channel="2" |
---|
1441 | type="float" |
---|
1442 | /> |
---|
1443 | <property |
---|
1444 | name="Cy5_Spotarea" |
---|
1445 | title="Cy5 spot area" |
---|
1446 | description="Area of spot in colour channel Cy5 in pixels." |
---|
1447 | column="cy5_area" |
---|
1448 | channel="2" |
---|
1449 | type="float" |
---|
1450 | /> |
---|
1451 | <property |
---|
1452 | name="Cy5_Satpixel" |
---|
1453 | title="Cy5 saturated pixels" |
---|
1454 | description="Number of saturated pixel in color channel Cy5." |
---|
1455 | column="cy5_saturated" |
---|
1456 | channel="2" |
---|
1457 | type="float" |
---|
1458 | /> |
---|
1459 | <property |
---|
1460 | name="Rep_vol_BG2" |
---|
1461 | title="Background reduced volume" |
---|
1462 | description="Average of background reduced volume for onchip replicas (when calculated)." |
---|
1463 | column="rep_vol" |
---|
1464 | type="float" |
---|
1465 | /> |
---|
1466 | <property |
---|
1467 | name="RatioCy5Cy3" |
---|
1468 | title="Direct ratio" |
---|
1469 | description="Direct ratio ( = Cy5_Vol-BG / Cy3_Vol-BG )." |
---|
1470 | column="ratio" |
---|
1471 | type="float" |
---|
1472 | /> |
---|
1473 | <property |
---|
1474 | name="Directional_RatioCy5Cy3" |
---|
1475 | title="Directional ratio" |
---|
1476 | description="Directional ratio: if Ratio > 1 then Directional = Ratio, if Ratio > 1 then Directional = -1/Ratio." |
---|
1477 | column="directional_ratio" |
---|
1478 | type="float" |
---|
1479 | /> |
---|
1480 | <property |
---|
1481 | name="Log2_RatioCy5Cy3" |
---|
1482 | title="Log2 ratio" |
---|
1483 | description="Log2 ratio: log2 (Ratio-Cy5/Cy3)" |
---|
1484 | column="log2_ratio" |
---|
1485 | type="float" |
---|
1486 | /> |
---|
1487 | <property |
---|
1488 | name="Comp_ChannelCy3" |
---|
1489 | title="Cy53 normalised intensity" |
---|
1490 | description="Normalised intensity of Cy3_Vol-BG." |
---|
1491 | column="cy3_normalised" |
---|
1492 | channel="1" |
---|
1493 | type="float" |
---|
1494 | /> |
---|
1495 | <property |
---|
1496 | name="Comp_ChannelCy5" |
---|
1497 | title="Cy5 normalised intensity" |
---|
1498 | description="Normalised intensity of Cy5_Vol-BG." |
---|
1499 | column="cy5_normalised" |
---|
1500 | channel="2" |
---|
1501 | type="float" |
---|
1502 | /> |
---|
1503 | <property |
---|
1504 | name="Comp_RatioCy5Cy3" |
---|
1505 | title="Compensated ratio" |
---|
1506 | description="Compensated ratio ( = Comp_Channel Cy5 / Comp_Channel Cy3 )." |
---|
1507 | column="compensated_ratio" |
---|
1508 | type="float" |
---|
1509 | /> |
---|
1510 | <property |
---|
1511 | name="Comp_Directional_RatioCy5Cy3" |
---|
1512 | title="Compensated directional ratio" |
---|
1513 | description="Compensated directional ratio: if Comp_Ratio > 1 then Directional = Ratio, if Ratio < 1 then Directional = -1/Ratio." |
---|
1514 | column="comp_dir_ratio" |
---|
1515 | type="float" |
---|
1516 | /> |
---|
1517 | <property |
---|
1518 | name="Comp_Log2_Ratio" |
---|
1519 | title="Compensated log2 ratio" |
---|
1520 | description="Compensated Log2 ratio: log2 (Comp_Ratio-Cy5/Cy3)." |
---|
1521 | column="comp_log2_ratio" |
---|
1522 | type="float" |
---|
1523 | /> |
---|
1524 | <property |
---|
1525 | name="Comment" |
---|
1526 | title="Comment" |
---|
1527 | description="Additional description of spot." |
---|
1528 | column="comment" |
---|
1529 | type="string" |
---|
1530 | /> |
---|
1531 | <property |
---|
1532 | name="SNRtot" |
---|
1533 | title="Squared average SNR" |
---|
1534 | description="Squared average of Cy5_SNR and Cy3_SNR." |
---|
1535 | column="snr_tot" |
---|
1536 | type="float" |
---|
1537 | /> |
---|
1538 | <property |
---|
1539 | name="SNRtot_Score" |
---|
1540 | title="Squared average score SNR" |
---|
1541 | description="Squared average of Cy5_%SNR and Cy3_%SNR." |
---|
1542 | column="snr_tot_score" |
---|
1543 | type="float" |
---|
1544 | /> |
---|
1545 | <property |
---|
1546 | name="SNRGS" |
---|
1547 | title="Saturated pixel" |
---|
1548 | description="Number of saturated pixel in both colors (logical or)." |
---|
1549 | column="sat_pixels" |
---|
1550 | type="float" |
---|
1551 | /> |
---|
1552 | <property |
---|
1553 | name="Replicas" |
---|
1554 | title="Replicas" |
---|
1555 | description="Replicas" |
---|
1556 | column="replicas" |
---|
1557 | type="float" |
---|
1558 | /> |
---|
1559 | <property |
---|
1560 | name="Regulation" |
---|
1561 | title="Regulation" |
---|
1562 | description="If Flag = SNR1 then Regulation=>Down; if Flag = SNR2 then Regulation=>up; if Flag = SNR1 AND SNR2 then Regulation=>>weak; else Regulation = Ratio-Cy5/Cy3." |
---|
1563 | column="regulation" |
---|
1564 | type="string" |
---|
1565 | /> |
---|
1566 | <property |
---|
1567 | name="GroupName" |
---|
1568 | title="Group name" |
---|
1569 | description="Group name for this spot." |
---|
1570 | column="group_name" |
---|
1571 | type="string" |
---|
1572 | /> |
---|
1573 | <property |
---|
1574 | name="MedianRatios" |
---|
1575 | title="Median of ratios" |
---|
1576 | description="Median of ratios (Cy5/Cy3) from all pixel in this spot." |
---|
1577 | column="median_ratios" |
---|
1578 | type="float" |
---|
1579 | /> |
---|
1580 | <property |
---|
1581 | name="MedianRatiosSDev" |
---|
1582 | title="Median of ratios (sdev%)" |
---|
1583 | description="Standard deviation of Median of ratios in percent." |
---|
1584 | column="median_ratios_sdev" |
---|
1585 | type="float" |
---|
1586 | /> |
---|
1587 | <property |
---|
1588 | name="RatioMedians" |
---|
1589 | title="Ratio of medians" |
---|
1590 | description="Ratio of Medians (Cy5/Cy3) from all pixel in this spot." |
---|
1591 | column="ratio_medians" |
---|
1592 | type="float" |
---|
1593 | /> |
---|
1594 | <property |
---|
1595 | name="RatioMediansSDev" |
---|
1596 | title="Ratio of medians (sdev%)" |
---|
1597 | description="Standard deviation of Ratio of medians in percent." |
---|
1598 | column="ratio_medians_sdev" |
---|
1599 | type="float" |
---|
1600 | /> |
---|
1601 | <intensity-formula |
---|
1602 | name="Volume" |
---|
1603 | title="Volume" |
---|
1604 | description="Total integrated signal intensity" |
---|
1605 | > |
---|
1606 | <formula |
---|
1607 | channel="1" |
---|
1608 | expression="raw('Cy3_Volume')" |
---|
1609 | /> |
---|
1610 | <formula |
---|
1611 | channel="2" |
---|
1612 | expression="raw('Cy5_Volume')" |
---|
1613 | /> |
---|
1614 | </intensity-formula> |
---|
1615 | <intensity-formula |
---|
1616 | name="VolBG" |
---|
1617 | title="Reduced intensity" |
---|
1618 | description="Background reduced intensity" |
---|
1619 | > |
---|
1620 | <formula |
---|
1621 | channel="1" |
---|
1622 | expression="raw('Cy3_VolBG')" |
---|
1623 | /> |
---|
1624 | <formula |
---|
1625 | channel="2" |
---|
1626 | expression="raw('Cy5_VolBG')" |
---|
1627 | /> |
---|
1628 | </intensity-formula> |
---|
1629 | </raw-data-type> |
---|
1630 | |
---|
1631 | <raw-data-type |
---|
1632 | id="genetac" |
---|
1633 | channels="2" |
---|
1634 | name="GeneTAC" |
---|
1635 | table="RawDataGeneTac" |
---|
1636 | > |
---|
1637 | <property |
---|
1638 | name="medCy3" |
---|
1639 | title="Signal median Cy3" |
---|
1640 | description="Median of Cy3 signal intensity." |
---|
1641 | column="med_cy3" |
---|
1642 | channel="1" |
---|
1643 | type="float" |
---|
1644 | /> |
---|
1645 | <property |
---|
1646 | name="medCy5" |
---|
1647 | title="Signal median Cy5" |
---|
1648 | description="Median of Cy5 signal intensity." |
---|
1649 | column="med_cy5" |
---|
1650 | channel="2" |
---|
1651 | type="float" |
---|
1652 | /> |
---|
1653 | <property |
---|
1654 | name="medRatio" |
---|
1655 | title="Signal median ratio" |
---|
1656 | description="Ratio of median signal." |
---|
1657 | column="med_ratio" |
---|
1658 | type="float" |
---|
1659 | /> |
---|
1660 | <property |
---|
1661 | name="bgCy3" |
---|
1662 | title="Background median Cy3" |
---|
1663 | description="Median of background intensity for Cy3." |
---|
1664 | column="bg_cy3" |
---|
1665 | channel="1" |
---|
1666 | type="float" |
---|
1667 | /> |
---|
1668 | <property |
---|
1669 | name="bgCy5" |
---|
1670 | title="Background median Cy5" |
---|
1671 | description="Median of background intensity for Cy5." |
---|
1672 | column="bg_cy5" |
---|
1673 | channel="2" |
---|
1674 | type="float" |
---|
1675 | /> |
---|
1676 | <property |
---|
1677 | name="bgRatio" |
---|
1678 | title="Signal med ratio" |
---|
1679 | description="Ratio of median background." |
---|
1680 | column="bg_ratio" |
---|
1681 | type="float" |
---|
1682 | /> |
---|
1683 | <intensity-formula |
---|
1684 | name="medianfg" |
---|
1685 | title="Median FG" |
---|
1686 | description="Median foreground" |
---|
1687 | > |
---|
1688 | <formula |
---|
1689 | channel="1" |
---|
1690 | expression="raw('medCy3')" |
---|
1691 | /> |
---|
1692 | <formula |
---|
1693 | channel="2" |
---|
1694 | expression="raw('medCy5')" |
---|
1695 | /> |
---|
1696 | </intensity-formula> |
---|
1697 | <intensity-formula |
---|
1698 | name="median" |
---|
1699 | title="Median FG - Median BG" |
---|
1700 | description="Subtract median background from median foreground" |
---|
1701 | > |
---|
1702 | <formula |
---|
1703 | channel="1" |
---|
1704 | expression="raw('medCy3') - raw('bgCy3')" |
---|
1705 | /> |
---|
1706 | <formula |
---|
1707 | channel="2" |
---|
1708 | expression="raw('medCy5') - raw('bgCy5')" |
---|
1709 | /> |
---|
1710 | </intensity-formula> |
---|
1711 | </raw-data-type> |
---|
1712 | |
---|
1713 | <raw-data-type |
---|
1714 | id="imagene" |
---|
1715 | channels="2" |
---|
1716 | name="ImaGene" |
---|
1717 | table="RawDataImaGene" |
---|
1718 | > |
---|
1719 | <property |
---|
1720 | name="Field" |
---|
1721 | title="Field" |
---|
1722 | description="Name of a field where the spot is located." |
---|
1723 | column="field" |
---|
1724 | type="string" |
---|
1725 | /> |
---|
1726 | <property |
---|
1727 | name="Flag_Cy3" |
---|
1728 | title="Flag cy3" |
---|
1729 | description="Numeric code for the spot (0 - no flag, flag codes 1,...,7)." |
---|
1730 | column="cy3_flag" |
---|
1731 | channel="1" |
---|
1732 | type="int" |
---|
1733 | /> |
---|
1734 | <property |
---|
1735 | name="SignalMean_Cy3" |
---|
1736 | title="Mean signal cy3" |
---|
1737 | description="Pixel intensity averaged over the local signal region." |
---|
1738 | column="cy3_mean_signal" |
---|
1739 | channel="1" |
---|
1740 | type="float" |
---|
1741 | /> |
---|
1742 | <property |
---|
1743 | name="BackgroundMean_Cy3" |
---|
1744 | title="Mean background cy3" |
---|
1745 | description="Pixel intensity averaged over the local background region." |
---|
1746 | column="cy3_mean_bg" |
---|
1747 | channel="1" |
---|
1748 | type="float" |
---|
1749 | /> |
---|
1750 | <property |
---|
1751 | name="SignalMedian_Cy3" |
---|
1752 | title="Median signal cy3" |
---|
1753 | description="Median pixel intensity computed over the local signal region." |
---|
1754 | column="cy3_median_signal" |
---|
1755 | channel="1" |
---|
1756 | type="float" |
---|
1757 | /> |
---|
1758 | <property |
---|
1759 | name="BackgroundMedian_Cy3" |
---|
1760 | title="Median background cy3" |
---|
1761 | description="Median pixel intensity averaged over the local background region." |
---|
1762 | column="cy3_median_bg" |
---|
1763 | channel="1" |
---|
1764 | type="float" |
---|
1765 | /> |
---|
1766 | <property |
---|
1767 | name="SignalMode_Cy3" |
---|
1768 | title="Mode signal cy3" |
---|
1769 | description="Mode pixel intensity computed over the local signal region (mode corresponds to the peak location in intensity distribution)." |
---|
1770 | column="cy3_mode_signal" |
---|
1771 | channel="1" |
---|
1772 | type="float" |
---|
1773 | /> |
---|
1774 | <property |
---|
1775 | name="BackgroundMode_Cy3" |
---|
1776 | title="Mode background cy3" |
---|
1777 | description="Mode pixel intensity computed over the local background region." |
---|
1778 | column="cy3_mode_bg" |
---|
1779 | channel="1" |
---|
1780 | type="float" |
---|
1781 | /> |
---|
1782 | <property |
---|
1783 | name="SignalArea_Cy3" |
---|
1784 | title="Signal area cy3" |
---|
1785 | description="Number of pixels in the local signal region." |
---|
1786 | column="cy3_area_signal" |
---|
1787 | channel="1" |
---|
1788 | type="int" |
---|
1789 | /> |
---|
1790 | <property |
---|
1791 | name="BackgroundArea_Cy3" |
---|
1792 | title="Background area cy3" |
---|
1793 | description="Number of pixels in the local background region." |
---|
1794 | column="cy3_area_bg" |
---|
1795 | channel="1" |
---|
1796 | type="int" |
---|
1797 | /> |
---|
1798 | <property |
---|
1799 | name="SignalTotal_Cy3" |
---|
1800 | title="Total signal cy3" |
---|
1801 | description="Total pixel intensity summed over the local signal region." |
---|
1802 | column="cy3_total_signal" |
---|
1803 | channel="1" |
---|
1804 | type="int" |
---|
1805 | /> |
---|
1806 | <property |
---|
1807 | name="BackgroundTotal_Cy3" |
---|
1808 | title="Total background cy3" |
---|
1809 | description="Total pixel intensity summed over the local background region." |
---|
1810 | column="cy3_total_bg" |
---|
1811 | channel="1" |
---|
1812 | type="int" |
---|
1813 | /> |
---|
1814 | <property |
---|
1815 | name="ShapeRegularity_Cy3" |
---|
1816 | title="Shape regularity cy3" |
---|
1817 | description="The signal area of a spot is inscribed into a circle, then the number of non-signal pixels that fall within this circle is computed and divided by area of circle. This ratio is subtracted from 1 to give the Shape Regularity." |
---|
1818 | column="cy3_shape" |
---|
1819 | channel="1" |
---|
1820 | type="float" |
---|
1821 | /> |
---|
1822 | <property |
---|
1823 | name="IgnoredArea_Cy3" |
---|
1824 | title="Ignored area cy3" |
---|
1825 | description="Area of ignored regions directly neighboring (touching) the signal area is computed." |
---|
1826 | column="cy3_area_ignored" |
---|
1827 | channel="1" |
---|
1828 | type="int" |
---|
1829 | /> |
---|
1830 | <property |
---|
1831 | name="SpotArea_Cy3" |
---|
1832 | title="Spot area cy3" |
---|
1833 | description="Signal Area plus Ignored Area." |
---|
1834 | column="cy3_area_spot" |
---|
1835 | channel="1" |
---|
1836 | type="int" |
---|
1837 | /> |
---|
1838 | <property |
---|
1839 | name="IgnoredMedian_Cy3" |
---|
1840 | title="Median ignored cy3" |
---|
1841 | description="Median pixel intensity computed over the local ignored region." |
---|
1842 | column="cy3_median_ignored" |
---|
1843 | channel="1" |
---|
1844 | type="float" |
---|
1845 | /> |
---|
1846 | <property |
---|
1847 | name="AreaToPerimeter_Cy3" |
---|
1848 | title="Area to perimeter cy3" |
---|
1849 | description="This quality measure defines the spot's circularity. Area of a spot is divided by a square of spot perimeter and multiplied by 4π. As a result, this measure ranges from 0 (highly non-circular shape) to 1 (a perfect circle)." |
---|
1850 | column="cy3_area_perimeter" |
---|
1851 | channel="1" |
---|
1852 | type="float" |
---|
1853 | /> |
---|
1854 | <property |
---|
1855 | name="OpenPerimeter_Cy3" |
---|
1856 | title="Open perimeter cy3" |
---|
1857 | description="Computes the proportion of signal perimeter that touches the border of rectangular snip around the spot." |
---|
1858 | column="cy3_open_perimeter" |
---|
1859 | channel="1" |
---|
1860 | type="float" |
---|
1861 | /> |
---|
1862 | <property |
---|
1863 | name="XCoord_Cy3" |
---|
1864 | title="X coordinate cy3" |
---|
1865 | description="X coordinate (in pixels) of grid circle corresponding to the spot." |
---|
1866 | column="cy3_x" |
---|
1867 | channel="1" |
---|
1868 | type="float" |
---|
1869 | /> |
---|
1870 | <property |
---|
1871 | name="YCoord_Cy3" |
---|
1872 | title="Y coordinate cy3" |
---|
1873 | description="Y coordinate (in pixels) of grid circle corresponding to the spot." |
---|
1874 | column="cy3_y" |
---|
1875 | channel="1" |
---|
1876 | type="float" |
---|
1877 | /> |
---|
1878 | <property |
---|
1879 | name="Diameter_Cy3" |
---|
1880 | title="Diameter cy3" |
---|
1881 | description="Diameter (in pixels) of grid circle corresponding to the spot." |
---|
1882 | column="cy3_diameter" |
---|
1883 | channel="1" |
---|
1884 | type="int" |
---|
1885 | /> |
---|
1886 | <property |
---|
1887 | name="PositionOffset_Cy3" |
---|
1888 | title="Position offset cy3" |
---|
1889 | description="Offset (in pixels) of the center of the grid circle from the expected position in the grid." |
---|
1890 | column="cy3_offset" |
---|
1891 | channel="1" |
---|
1892 | type="float" |
---|
1893 | /> |
---|
1894 | <property |
---|
1895 | name="OffsetX_Cy3" |
---|
1896 | title="X offset cy3" |
---|
1897 | description="X Offset (in pixels) of the center of the grid circle from the expected position in the grid." |
---|
1898 | column="cy3_offset_x" |
---|
1899 | channel="1" |
---|
1900 | type="float" |
---|
1901 | /> |
---|
1902 | <property |
---|
1903 | name="OffsetY_Cy3" |
---|
1904 | title="Y offset cy3" |
---|
1905 | description="Y Offset (in pixels) of the center of the grid circle from the expected position in the grid." |
---|
1906 | column="cy3_offset_y" |
---|
1907 | channel="1" |
---|
1908 | type="float" |
---|
1909 | /> |
---|
1910 | <property |
---|
1911 | name="ExpectedX_Cy3" |
---|
1912 | title="X expected cy3" |
---|
1913 | description="X coordinate of expected position of the circle in the grid. Expected position in the grid is computed fitting least square lines to circle centers in every row and column." |
---|
1914 | column="cy3_x_expected" |
---|
1915 | channel="1" |
---|
1916 | type="float" |
---|
1917 | /> |
---|
1918 | <property |
---|
1919 | name="ExpectedY_Cy3" |
---|
1920 | title="Y expected cy3" |
---|
1921 | description="Y coordinate of expected position of the circle in the grid. Expected position in the grid is computed fitting least square lines to circle centers in every row and column." |
---|
1922 | column="cy3_y_expected" |
---|
1923 | channel="1" |
---|
1924 | type="float" |
---|
1925 | /> |
---|
1926 | <property |
---|
1927 | name="CMX_Cy3" |
---|
1928 | title="CM X coordinate cy3" |
---|
1929 | description="X coordinate of the center of the mass of spot's signal region." |
---|
1930 | column="cy3_x_cm" |
---|
1931 | channel="1" |
---|
1932 | type="float" |
---|
1933 | /> |
---|
1934 | <property |
---|
1935 | name="CMY_Cy3" |
---|
1936 | title="CM Y coordinate cy3" |
---|
1937 | description="Y coordinate of the center of the mass of spot's signal region." |
---|
1938 | column="cy3_y_cm" |
---|
1939 | channel="1" |
---|
1940 | type="float" |
---|
1941 | /> |
---|
1942 | <property |
---|
1943 | name="CMOffset_Cy3" |
---|
1944 | title="CM offset cy3" |
---|
1945 | description="Offset (in pixels) of the spot's center of the mass from the expected position in the grid." |
---|
1946 | column="cy3_offset_cm" |
---|
1947 | channel="1" |
---|
1948 | type="float" |
---|
1949 | /> |
---|
1950 | <property |
---|
1951 | name="CMOffsetX_Cy3" |
---|
1952 | title="CM X offset cy3" |
---|
1953 | description="X offset (in pixels) of the spot's center of the mass from the expected position in the grid." |
---|
1954 | column="cy3_offset_x_cm" |
---|
1955 | channel="1" |
---|
1956 | type="float" |
---|
1957 | /> |
---|
1958 | <property |
---|
1959 | name="CMOffsetY_Cy3" |
---|
1960 | title="CM Y offset cy3" |
---|
1961 | description="Y offset (in pixels) of the spot's center of the mass from the expected position in the grid." |
---|
1962 | column="cy3_offset_y_cm" |
---|
1963 | channel="1" |
---|
1964 | type="float" |
---|
1965 | /> |
---|
1966 | <property |
---|
1967 | name="MinDiam_Cy3" |
---|
1968 | title="Min diameter cy3" |
---|
1969 | description="Diameter of the circle inscribed into the spot's signal region." |
---|
1970 | column="cy3_diameter_min" |
---|
1971 | channel="1" |
---|
1972 | type="float" |
---|
1973 | /> |
---|
1974 | <property |
---|
1975 | name="MaxDiam_Cy3" |
---|
1976 | title="Max diameter cy3" |
---|
1977 | description="Diameter of the circle, the spot's signal region can be inscribed in." |
---|
1978 | column="cy3_diameter_max" |
---|
1979 | channel="1" |
---|
1980 | type="float" |
---|
1981 | /> |
---|
1982 | <property |
---|
1983 | name="Control_Cy3" |
---|
1984 | title="Control cy3" |
---|
1985 | description="Name of a control type for current spot (no name means the spot is not a control spot)." |
---|
1986 | column="cy3_control" |
---|
1987 | channel="1" |
---|
1988 | type="string" |
---|
1989 | /> |
---|
1990 | <property |
---|
1991 | name="FailedControl_Cy3" |
---|
1992 | title="Failed control cy3" |
---|
1993 | description="0 if the control passed all tests, 1 if at least one of the tests failed." |
---|
1994 | column="cy3_control_failed" |
---|
1995 | channel="1" |
---|
1996 | type="boolean" |
---|
1997 | /> |
---|
1998 | <property |
---|
1999 | name="BackgroundContaminationPresent_Cy3" |
---|
2000 | title="Bg contamination cy3" |
---|
2001 | description="0 if the spot passed background contamination test, 1 if it did not." |
---|
2002 | column="cy3_bg_contamination" |
---|
2003 | channel="1" |
---|
2004 | type="boolean" |
---|
2005 | /> |
---|
2006 | <property |
---|
2007 | name="SignalContaminationPresent_Cy3" |
---|
2008 | title="Signal contamination cy3" |
---|
2009 | description="0 if the spot passed signal contamination test, 1 if it did not." |
---|
2010 | column="cy3_signal_contamination" |
---|
2011 | channel="1" |
---|
2012 | type="boolean" |
---|
2013 | /> |
---|
2014 | <property |
---|
2015 | name="IgnoredFailed_Cy3" |
---|
2016 | title="Ignored % failed cy3" |
---|
2017 | description="0 if the spot passed ignored percentage test, 1 if it did not." |
---|
2018 | column="cy3_ignored_failed" |
---|
2019 | channel="1" |
---|
2020 | type="boolean" |
---|
2021 | /> |
---|
2022 | <property |
---|
2023 | name="OpenPerimeterFailed_Cy3" |
---|
2024 | title="Open perimeter failed cy3" |
---|
2025 | description="0 if the spot passed open perimeter test, 1 if it did not." |
---|
2026 | column="cy3_perimeter_failed" |
---|
2027 | channel="1" |
---|
2028 | type="boolean" |
---|
2029 | /> |
---|
2030 | <property |
---|
2031 | name="ShapeRegularityFailed_Cy3" |
---|
2032 | title="Shape regularity failed cy3" |
---|
2033 | description="0 if the spot passed shape regularity test, 1 if it did not." |
---|
2034 | column="cy3_shape_failed" |
---|
2035 | channel="1" |
---|
2036 | type="boolean" |
---|
2037 | /> |
---|
2038 | <property |
---|
2039 | name="PerimToAreaFailed_Cy3" |
---|
2040 | title="Perimeter to area failed cy3" |
---|
2041 | description="0 if the spot passed perimeter-to-area test, 1 if it did not." |
---|
2042 | column="cy3_areapm_failed" |
---|
2043 | channel="1" |
---|
2044 | type="boolean" |
---|
2045 | /> |
---|
2046 | <property |
---|
2047 | name="OffsetFailed_Cy3" |
---|
2048 | title="Offset failed cy3" |
---|
2049 | description="0 if the spot passed offset test, 1 if it did not." |
---|
2050 | column="cy3_offset_failed" |
---|
2051 | channel="1" |
---|
2052 | type="boolean" |
---|
2053 | /> |
---|
2054 | <property |
---|
2055 | name="EmptySpot_Cy3" |
---|
2056 | title="Empty spot cy3" |
---|
2057 | description="1 if the spot is empty, 0 if not." |
---|
2058 | column="cy3_empty_spot" |
---|
2059 | channel="1" |
---|
2060 | type="boolean" |
---|
2061 | /> |
---|
2062 | <property |
---|
2063 | name="NegativeSpot_Cy3" |
---|
2064 | title="Negative spot cy3" |
---|
2065 | description="1 if the spot was qualified as negative, 0 if it was not." |
---|
2066 | column="cy3_negative_spot" |
---|
2067 | channel="1" |
---|
2068 | type="boolean" |
---|
2069 | /> |
---|
2070 | <property |
---|
2071 | name="SelectedSpot_Cy3" |
---|
2072 | title="Selected spot cy3" |
---|
2073 | description="1 if the spot was selected in the software view at time of save, 0 if not." |
---|
2074 | column="cy3_selected_spot" |
---|
2075 | channel="1" |
---|
2076 | type="boolean" |
---|
2077 | /> |
---|
2078 | <property |
---|
2079 | name="SaturatedSpot_Cy3" |
---|
2080 | title="Saturated spot cy3" |
---|
2081 | description="1 if the spot was qualified as saturated, 0 if it was not." |
---|
2082 | column="cy3_saturated_spot" |
---|
2083 | channel="1" |
---|
2084 | type="boolean" |
---|
2085 | /> |
---|
2086 | <property |
---|
2087 | name="SignalMean_Cy5" |
---|
2088 | title="Mean signal cy5" |
---|
2089 | description="Pixel intensity averaged over the local signal region." |
---|
2090 | column="cy5_mean_signal" |
---|
2091 | channel="2" |
---|
2092 | type="float" |
---|
2093 | /> |
---|
2094 | <property |
---|
2095 | name="BackgroundMean_Cy5" |
---|
2096 | title="Mean background cy5" |
---|
2097 | description="Pixel intensity averaged over the local background region." |
---|
2098 | column="cy5_mean_bg" |
---|
2099 | channel="2" |
---|
2100 | type="float" |
---|
2101 | /> |
---|
2102 | <property |
---|
2103 | name="SignalMedian_Cy5" |
---|
2104 | title="Median signal cy5" |
---|
2105 | description="Median pixel intensity computed over the local signal region." |
---|
2106 | column="cy5_median_signal" |
---|
2107 | channel="2" |
---|
2108 | type="float" |
---|
2109 | /> |
---|
2110 | <property |
---|
2111 | name="BackgroundMedian_Cy5" |
---|
2112 | title="Median background cy5" |
---|
2113 | description="Median pixel intensity averaged over the local background region." |
---|
2114 | column="cy5_median_bg" |
---|
2115 | channel="2" |
---|
2116 | type="float" |
---|
2117 | /> |
---|
2118 | <property |
---|
2119 | name="SignalMode_Cy5" |
---|
2120 | title="Mode signal cy5" |
---|
2121 | description="Mode pixel intensity computed over the local signal region (mode corresponds to the peak location in intensity distribution)." |
---|
2122 | column="cy5_mode_signal" |
---|
2123 | channel="2" |
---|
2124 | type="float" |
---|
2125 | /> |
---|
2126 | <property |
---|
2127 | name="BackgroundMode_Cy5" |
---|
2128 | title="Mode background cy5" |
---|
2129 | description="Mode pixel intensity computed over the local background region." |
---|
2130 | column="cy5_mode_bg" |
---|
2131 | channel="2" |
---|
2132 | type="float" |
---|
2133 | /> |
---|
2134 | <property |
---|
2135 | name="SignalArea_Cy5" |
---|
2136 | title="Signal area cy5" |
---|
2137 | description="Number of pixels in the local signal region." |
---|
2138 | column="cy5_area_signal" |
---|
2139 | channel="2" |
---|
2140 | type="int" |
---|
2141 | /> |
---|
2142 | <property |
---|
2143 | name="BackgroundArea_Cy5" |
---|
2144 | title="Background area cy5" |
---|
2145 | description="Number of pixels in the local background region." |
---|
2146 | column="cy5_area_bg" |
---|
2147 | channel="2" |
---|
2148 | type="int" |
---|
2149 | /> |
---|
2150 | <property |
---|
2151 | name="SignalTotal_Cy5" |
---|
2152 | title="Total signal cy5" |
---|
2153 | description="Total pixel intensity summed over the local signal region." |
---|
2154 | column="cy5_total_signal" |
---|
2155 | channel="2" |
---|
2156 | type="int" |
---|
2157 | /> |
---|
2158 | <property |
---|
2159 | name="BackgroundTotal_Cy5" |
---|
2160 | title="Total background cy5" |
---|
2161 | description="Total pixel intensity summed over the local background region." |
---|
2162 | column="cy5_total_bg" |
---|
2163 | channel="2" |
---|
2164 | type="int" |
---|
2165 | /> |
---|
2166 | <property |
---|
2167 | name="ShapeRegularity_Cy5" |
---|
2168 | title="Shape regularity cy5" |
---|
2169 | description="The signal area of a spot is inscribed into a circle, then the number of non-signal pixels that fall within this circle is computed and divided by area of circle. This ratio is subtracted from 1 to give the Shape Regularity." |
---|
2170 | column="cy5_shape" |
---|
2171 | channel="2" |
---|
2172 | type="float" |
---|
2173 | /> |
---|
2174 | <property |
---|
2175 | name="IgnoredArea_Cy5" |
---|
2176 | title="Ignored area cy5" |
---|
2177 | description="Area of ignored regions directly neighboring (touching) the signal area is computed." |
---|
2178 | column="cy5_area_ignored" |
---|
2179 | channel="2" |
---|
2180 | type="int" |
---|
2181 | /> |
---|
2182 | <property |
---|
2183 | name="SpotArea_Cy5" |
---|
2184 | title="Spot area cy5" |
---|
2185 | description="Signal Area plus Ignored Area." |
---|
2186 | column="cy5_area_spot" |
---|
2187 | channel="2" |
---|
2188 | type="int" |
---|
2189 | /> |
---|
2190 | <property |
---|
2191 | name="IgnoredMedian_Cy5" |
---|
2192 | title="Median ignored cy5" |
---|
2193 | description="Median pixel intensity computed over the local ignored region." |
---|
2194 | column="cy5_median_ignored" |
---|
2195 | channel="2" |
---|
2196 | type="float" |
---|
2197 | /> |
---|
2198 | <property |
---|
2199 | name="AreaToPerimeter_Cy5" |
---|
2200 | title="Area to perimeter cy5" |
---|
2201 | description="This quality measure defines the spot's circularity. Area of a spot is divided by a square of spot perimeter and multiplied by 4π. As a result, this measure ranges from 0 (highly non-circular shape) to 1 (a perfect circle)." |
---|
2202 | column="cy5_area_perimeter" |
---|
2203 | channel="2" |
---|
2204 | type="float" |
---|
2205 | /> |
---|
2206 | <property |
---|
2207 | name="OpenPerimeter_Cy5" |
---|
2208 | title="Open perimeter cy5" |
---|
2209 | description="Computes the proportion of signal perimeter that touches the border of rectangular snip around the spot." |
---|
2210 | column="cy5_open_perimeter" |
---|
2211 | channel="2" |
---|
2212 | type="float" |
---|
2213 | /> |
---|
2214 | <property |
---|
2215 | name="XCoord_Cy5" |
---|
2216 | title="X coordinate cy5" |
---|
2217 | description="X coordinate (in pixels) of grid circle corresponding to the spot." |
---|
2218 | column="cy5_x" |
---|
2219 | channel="2" |
---|
2220 | type="float" |
---|
2221 | /> |
---|
2222 | <property |
---|
2223 | name="YCoord_Cy5" |
---|
2224 | title="Y coordinate cy5" |
---|
2225 | description="Y coordinate (in pixels) of grid circle corresponding to the spot." |
---|
2226 | column="cy5_y" |
---|
2227 | channel="2" |
---|
2228 | type="float" |
---|
2229 | /> |
---|
2230 | <property |
---|
2231 | name="Diameter_Cy5" |
---|
2232 | title="Diameter cy5" |
---|
2233 | description="Diameter (in pixels) of grid circle corresponding to the spot." |
---|
2234 | column="cy5_diameter" |
---|
2235 | channel="2" |
---|
2236 | type="int" |
---|
2237 | /> |
---|
2238 | <property |
---|
2239 | name="PositionOffset_Cy5" |
---|
2240 | title="Position offset cy5" |
---|
2241 | description="Offset (in pixels) of the center of the grid circle from the expected position in the grid." |
---|
2242 | column="cy5_offset" |
---|
2243 | channel="2" |
---|
2244 | type="float" |
---|
2245 | /> |
---|
2246 | <property |
---|
2247 | name="OffsetX_Cy5" |
---|
2248 | title="X offset cy5" |
---|
2249 | description="X Offset (in pixels) of the center of the grid circle from the expected position in the grid." |
---|
2250 | column="cy5_offset_x" |
---|
2251 | channel="2" |
---|
2252 | type="float" |
---|
2253 | /> |
---|
2254 | <property |
---|
2255 | name="OffsetY_Cy5" |
---|
2256 | title="Y offset cy5" |
---|
2257 | description="Y Offset (in pixels) of the center of the grid circle from the expected position in the grid." |
---|
2258 | column="cy5_offset_y" |
---|
2259 | channel="2" |
---|
2260 | type="float" |
---|
2261 | /> |
---|
2262 | <property |
---|
2263 | name="ExpectedX_Cy5" |
---|
2264 | title="X expected cy5" |
---|
2265 | description="X coordinate of expected position of the circle in the grid. Expected position in the grid is computed fitting least square lines to circle centers in every row and column." |
---|
2266 | column="cy5_x_expected" |
---|
2267 | channel="2" |
---|
2268 | type="float" |
---|
2269 | /> |
---|
2270 | <property |
---|
2271 | name="ExpectedY_Cy5" |
---|
2272 | title="Y expected cy5" |
---|
2273 | description="Y coordinate of expected position of the circle in the grid. Expected position in the grid is computed fitting least square lines to circle centers in every row and column." |
---|
2274 | column="cy5_y_expected" |
---|
2275 | channel="2" |
---|
2276 | type="float" |
---|
2277 | /> |
---|
2278 | <property |
---|
2279 | name="CMX_Cy5" |
---|
2280 | title="CM X coordinate cy5" |
---|
2281 | description="X coordinate of the center of the mass of spot's signal region." |
---|
2282 | column="cy5_x_cm" |
---|
2283 | channel="2" |
---|
2284 | type="float" |
---|
2285 | /> |
---|
2286 | <property |
---|
2287 | name="CMY_Cy5" |
---|
2288 | title="CM Y coordinate cy5" |
---|
2289 | description="Y coordinate of the center of the mass of spot's signal region." |
---|
2290 | column="cy5_y_cm" |
---|
2291 | channel="2" |
---|
2292 | type="float" |
---|
2293 | /> |
---|
2294 | <property |
---|
2295 | name="CMOffset_Cy5" |
---|
2296 | title="CM offset cy5" |
---|
2297 | description="Offset (in pixels) of the spot's center of the mass from the expected position in the grid." |
---|
2298 | column="cy5_offset_cm" |
---|
2299 | channel="2" |
---|
2300 | type="float" |
---|
2301 | /> |
---|
2302 | <property |
---|
2303 | name="CMOffsetX_Cy5" |
---|
2304 | title="CM X offset cy5" |
---|
2305 | description="X offset (in pixels) of the spot's center of the mass from the expected position in the grid." |
---|
2306 | column="cy5_offset_x_cm" |
---|
2307 | channel="2" |
---|
2308 | type="float" |
---|
2309 | /> |
---|
2310 | <property |
---|
2311 | name="CMOffsetY_Cy5" |
---|
2312 | title="CM Y offset cy5" |
---|
2313 | description="Y offset (in pixels) of the spot's center of the mass from the expected position in the grid." |
---|
2314 | column="cy5_offset_y_cm" |
---|
2315 | channel="2" |
---|
2316 | type="float" |
---|
2317 | /> |
---|
2318 | <property |
---|
2319 | name="MinDiam_Cy5" |
---|
2320 | title="Min diameter cy5" |
---|
2321 | description="Diameter of the circle inscribed into the spot's signal region." |
---|
2322 | column="cy5_diameter_min" |
---|
2323 | channel="2" |
---|
2324 | type="float" |
---|
2325 | /> |
---|
2326 | <property |
---|
2327 | name="MaxDiam_Cy5" |
---|
2328 | title="Max diameter cy5" |
---|
2329 | description="Diameter of the circle, the spot's signal region can be inscribed in." |
---|
2330 | column="cy5_diameter_max" |
---|
2331 | channel="2" |
---|
2332 | type="float" |
---|
2333 | /> |
---|
2334 | <property |
---|
2335 | name="Control_Cy5" |
---|
2336 | title="Control cy5" |
---|
2337 | description="Name of a control type for current spot (no name means the spot is not a control spot)." |
---|
2338 | column="cy5_control" |
---|
2339 | channel="2" |
---|
2340 | type="string" |
---|
2341 | /> |
---|
2342 | <property |
---|
2343 | name="FailedControl_Cy5" |
---|
2344 | title="Failed control cy5" |
---|
2345 | description="0 if the control passed all tests, 1 if at least one of the tests failed." |
---|
2346 | column="cy5_control_failed" |
---|
2347 | channel="2" |
---|
2348 | type="boolean" |
---|
2349 | /> |
---|
2350 | <property |
---|
2351 | name="BackgroundContaminationPresent_Cy5" |
---|
2352 | title="Bg contamination cy5" |
---|
2353 | description="0 if the spot passed background contamination test, 1 if it did not." |
---|
2354 | column="cy5_bg_contamination" |
---|
2355 | channel="2" |
---|
2356 | type="boolean" |
---|
2357 | /> |
---|
2358 | <property |
---|
2359 | name="SignalContaminationPresent_Cy5" |
---|
2360 | title="Signal contamination cy5" |
---|
2361 | description="0 if the spot passed signal contamination test, 1 if it did not." |
---|
2362 | column="cy5_signal_contamination" |
---|
2363 | channel="2" |
---|
2364 | type="boolean" |
---|
2365 | /> |
---|
2366 | <property |
---|
2367 | name="IgnoredFailed_Cy5" |
---|
2368 | title="Ignored % failed cy5" |
---|
2369 | description="0 if the spot passed ignored percentage test, 1 if it did not." |
---|
2370 | column="cy5_ignored_failed" |
---|
2371 | channel="2" |
---|
2372 | type="boolean" |
---|
2373 | /> |
---|
2374 | <property |
---|
2375 | name="OpenPerimeterFailed_Cy5" |
---|
2376 | title="Open perimeter failed cy5" |
---|
2377 | description="0 if the spot passed open perimeter test, 1 if it did not." |
---|
2378 | column="cy5_perimeter_failed" |
---|
2379 | channel="2" |
---|
2380 | type="boolean" |
---|
2381 | /> |
---|
2382 | <property |
---|
2383 | name="ShapeRegularityFailed_Cy5" |
---|
2384 | title="Shape regularity failed cy5" |
---|
2385 | description="0 if the spot passed shape regularity test, 1 if it did not." |
---|
2386 | column="cy5_shape_failed" |
---|
2387 | channel="2" |
---|
2388 | type="boolean" |
---|
2389 | /> |
---|
2390 | <property |
---|
2391 | name="PerimToAreaFailed_Cy5" |
---|
2392 | title="Perimeter to area failed cy5" |
---|
2393 | description="0 if the spot passed perimeter-to-area test, 1 if it did not." |
---|
2394 | column="cy5_areapm_failed" |
---|
2395 | channel="2" |
---|
2396 | type="boolean" |
---|
2397 | /> |
---|
2398 | <property |
---|
2399 | name="OffsetFailed_Cy5" |
---|
2400 | title="Offset failed cy5" |
---|
2401 | description="0 if the spot passed offset test, 1 if it did not." |
---|
2402 | column="cy5_offset_failed" |
---|
2403 | channel="2" |
---|
2404 | type="boolean" |
---|
2405 | /> |
---|
2406 | <property |
---|
2407 | name="EmptySpot_Cy5" |
---|
2408 | title="Empty spot cy5" |
---|
2409 | description="1 if the spot is empty, 0 if not." |
---|
2410 | column="cy5_empty_spot" |
---|
2411 | channel="2" |
---|
2412 | type="boolean" |
---|
2413 | /> |
---|
2414 | <property |
---|
2415 | name="NegativeSpot_Cy5" |
---|
2416 | title="Negative spot cy5" |
---|
2417 | description="1 if the spot was qualified as negative, 0 if it was not." |
---|
2418 | column="cy5_negative_spot" |
---|
2419 | channel="2" |
---|
2420 | type="boolean" |
---|
2421 | /> |
---|
2422 | <property |
---|
2423 | name="SelectedSpot_Cy5" |
---|
2424 | title="Selected spot cy5" |
---|
2425 | description="1 if the spot was selected in the software view at time of save, 0 if not." |
---|
2426 | column="cy5_selected_spot" |
---|
2427 | channel="2" |
---|
2428 | type="boolean" |
---|
2429 | /> |
---|
2430 | <property |
---|
2431 | name="SaturatedSpot_Cy5" |
---|
2432 | title="Saturated spot cy5" |
---|
2433 | description="1 if the spot was qualified as saturated, 0 if it was not." |
---|
2434 | column="cy5_saturated_spot" |
---|
2435 | channel="2" |
---|
2436 | type="boolean" |
---|
2437 | /> |
---|
2438 | <intensity-formula |
---|
2439 | name="mean" |
---|
2440 | title="Mean FG - Mean BG" |
---|
2441 | description="Subtract mean background from mean foreground" |
---|
2442 | > |
---|
2443 | <formula |
---|
2444 | channel="1" |
---|
2445 | expression="raw('SignalMean_Cy3') - raw('BackgroundMean_Cy3')" |
---|
2446 | /> |
---|
2447 | <formula |
---|
2448 | channel="2" |
---|
2449 | expression="raw('SignalMean_Cy5') - raw('BackgroundMean_Cy5')" |
---|
2450 | /> |
---|
2451 | </intensity-formula> |
---|
2452 | <intensity-formula |
---|
2453 | name="median" |
---|
2454 | title="Median FG - Median BG" |
---|
2455 | description="Subtract meadian background from median foreground" |
---|
2456 | > |
---|
2457 | <formula |
---|
2458 | channel="1" |
---|
2459 | expression="raw('SignalMedian_Cy3') - raw('BackgroundMedian_Cy3')" |
---|
2460 | /> |
---|
2461 | <formula |
---|
2462 | channel="2" |
---|
2463 | expression="raw('SignalMedian_Cy5') - raw('BackgroundMedian_Cy5')" |
---|
2464 | /> |
---|
2465 | </intensity-formula> |
---|
2466 | <intensity-formula |
---|
2467 | name="medianmean" |
---|
2468 | title="Median FG - Mean BG" |
---|
2469 | description="Subtract mean background from median foreground" |
---|
2470 | > |
---|
2471 | <formula |
---|
2472 | channel="1" |
---|
2473 | expression="raw('SignalMedian_Cy3') - raw('BackgroundMean_Cy3')" |
---|
2474 | /> |
---|
2475 | <formula |
---|
2476 | channel="2" |
---|
2477 | expression="raw('SignalMedian_Cy5') - raw('BackgroundMean_Cy5')" |
---|
2478 | /> |
---|
2479 | </intensity-formula> |
---|
2480 | <intensity-formula |
---|
2481 | name="meanmedian" |
---|
2482 | title="Mean FG - Median BG" |
---|
2483 | description="Subtract median background from mean foreground" |
---|
2484 | > |
---|
2485 | <formula |
---|
2486 | channel="1" |
---|
2487 | expression="raw('SignalMean_Cy3') - raw('BackgroundMedian_Cy3')" |
---|
2488 | /> |
---|
2489 | <formula |
---|
2490 | channel="2" |
---|
2491 | expression="raw('SignalMean_Cy5') - raw('BackgroundMedian_Cy5')" |
---|
2492 | /> |
---|
2493 | </intensity-formula> |
---|
2494 | <intensity-formula |
---|
2495 | name="meanfg" |
---|
2496 | title="Mean FG" |
---|
2497 | description="Mean foreground" |
---|
2498 | > |
---|
2499 | <formula |
---|
2500 | channel="1" |
---|
2501 | expression="raw('SignalMean_Cy3')" |
---|
2502 | /> |
---|
2503 | <formula |
---|
2504 | channel="2" |
---|
2505 | expression="raw('SignalMean_Cy5')" |
---|
2506 | /> |
---|
2507 | </intensity-formula> |
---|
2508 | <intensity-formula |
---|
2509 | name="medianfg" |
---|
2510 | title="Median FG" |
---|
2511 | description="Median foreground" |
---|
2512 | > |
---|
2513 | <formula |
---|
2514 | channel="1" |
---|
2515 | expression="raw('SignalMedian_Cy3')" |
---|
2516 | /> |
---|
2517 | <formula |
---|
2518 | channel="2" |
---|
2519 | expression="raw('SignalMedian_Cy5')" |
---|
2520 | /> |
---|
2521 | </intensity-formula> |
---|
2522 | <intensity-formula |
---|
2523 | name="medianfgmeanmedianbg" |
---|
2524 | title="Median FG - Mean(Median BG)" |
---|
2525 | description="Subtract mean of median backgrounds from median foreground" |
---|
2526 | > |
---|
2527 | <formula |
---|
2528 | channel="1" |
---|
2529 | expression="raw('SignalMedian_Cy3') - mean('BackgroundMedian_Cy3')" |
---|
2530 | /> |
---|
2531 | <formula |
---|
2532 | channel="2" |
---|
2533 | expression="raw('SignalMedian_Cy5') - mean('BackgroundMedian_Cy5')" |
---|
2534 | /> |
---|
2535 | </intensity-formula> |
---|
2536 | <intensity-formula |
---|
2537 | name="meanfgmeanmeanbg" |
---|
2538 | title="Mean FG - Mean(Mean BG)" |
---|
2539 | description="Subtract mean of mean backgrounds from mean foreground" |
---|
2540 | > |
---|
2541 | <formula |
---|
2542 | channel="1" |
---|
2543 | expression="raw('SignalMean_Cy3') - mean('BackgroundMean_Cy3')" |
---|
2544 | /> |
---|
2545 | <formula |
---|
2546 | channel="2" |
---|
2547 | expression="raw('SignalMean_Cy5') - mean('BackgroundMean_Cy5')" |
---|
2548 | /> |
---|
2549 | </intensity-formula> |
---|
2550 | <intensity-formula |
---|
2551 | name="medianfgmeanmeanbg" |
---|
2552 | title="Median FG - Mean(Mean BG)" |
---|
2553 | description="Subtract mean of mean backgrounds from median foreground" |
---|
2554 | > |
---|
2555 | <formula |
---|
2556 | channel="1" |
---|
2557 | expression="raw('SignalMedian_Cy3') - mean('BackgroundMean_Cy3')" |
---|
2558 | /> |
---|
2559 | <formula |
---|
2560 | channel="2" |
---|
2561 | expression="raw('SignalMedian_Cy5') - mean('BackgroundMean_Cy5')" |
---|
2562 | /> |
---|
2563 | </intensity-formula> |
---|
2564 | <intensity-formula |
---|
2565 | name="meanfgmeanmedianbg" |
---|
2566 | title="Mean FG - Mean(Median BG)" |
---|
2567 | description="Subtract mean of median backgrounds from mean foreground" |
---|
2568 | > |
---|
2569 | <formula |
---|
2570 | channel="1" |
---|
2571 | expression="raw('SignalMean_Cy3') - mean('BackgroundMedian_Cy3')" |
---|
2572 | /> |
---|
2573 | <formula |
---|
2574 | channel="2" |
---|
2575 | expression="raw('SignalMean_Cy5') - mean('BackgroundMedian_Cy5')" |
---|
2576 | /> |
---|
2577 | </intensity-formula> |
---|
2578 | </raw-data-type> |
---|
2579 | |
---|
2580 | <raw-data-type |
---|
2581 | id="quantarraybiotin" |
---|
2582 | channels="1" |
---|
2583 | name="QuantArray Biotin" |
---|
2584 | table="RawDataQuantArrayBiotin" |
---|
2585 | > |
---|
2586 | <property |
---|
2587 | name="Number" |
---|
2588 | title="Number" |
---|
2589 | description="Number." |
---|
2590 | column="number" |
---|
2591 | type="int" |
---|
2592 | /> |
---|
2593 | <property |
---|
2594 | name="BiotinIntensity" |
---|
2595 | title="Intensity" |
---|
2596 | description="Mean pixel biotin foreground intensity in biotin channel." |
---|
2597 | column="intensity" |
---|
2598 | channel="1" |
---|
2599 | type="float" |
---|
2600 | /> |
---|
2601 | <property |
---|
2602 | name="BiotinBackground" |
---|
2603 | title="Background" |
---|
2604 | description="Mean pixel biotin background intensity in biotin channel." |
---|
2605 | column="background" |
---|
2606 | channel="1" |
---|
2607 | type="float" |
---|
2608 | /> |
---|
2609 | <property |
---|
2610 | name="BiotinDiameter" |
---|
2611 | title="Diameter" |
---|
2612 | description="Average diameter of the spot (in microns). Used to determine whether the spot is close to the expected size or is perhaps an artifact on the slide." |
---|
2613 | column="diameter" |
---|
2614 | channel="1" |
---|
2615 | type="float" |
---|
2616 | /> |
---|
2617 | <property |
---|
2618 | name="BiotinArea" |
---|
2619 | title="Area" |
---|
2620 | description="The area of the spot given as the number of pixels (in sq. microns). Used to determine whether the spot is close to the expected size or is an artifact on the slide." |
---|
2621 | column="area" |
---|
2622 | channel="1" |
---|
2623 | type="int" |
---|
2624 | /> |
---|
2625 | <property |
---|
2626 | name="BiotinFootprint" |
---|
2627 | title="Footprint" |
---|
2628 | description="The distance between the expected position of a spot and its actual measured position (in microns)." |
---|
2629 | column="footprint" |
---|
2630 | channel="1" |
---|
2631 | type="float" |
---|
2632 | /> |
---|
2633 | <property |
---|
2634 | name="BiotinCircularity" |
---|
2635 | title="Circularity" |
---|
2636 | description="Reports the degree to which a spot is close to a perfect circle. This measurement is used to determine the print quality of the microarray." |
---|
2637 | column="cirularity" |
---|
2638 | channel="1" |
---|
2639 | type="float" |
---|
2640 | /> |
---|
2641 | <property |
---|
2642 | name="BiotinSpotUniformity" |
---|
2643 | title="Spot uniformity" |
---|
2644 | description="The uniformity of the pixels used in the spot foreground intensity calculation. This helps in determining the quality of the spot and aids in selecting the correct quantification output method." |
---|
2645 | column="uniformity" |
---|
2646 | channel="1" |
---|
2647 | type="float" |
---|
2648 | /> |
---|
2649 | <property |
---|
2650 | name="BiotinBkgUniformity" |
---|
2651 | title="Background uniformity" |
---|
2652 | description="The uniformity of the pixels used in the background intensity calculation." |
---|
2653 | column="bg_uniformity" |
---|
2654 | channel="1" |
---|
2655 | type="float" |
---|
2656 | /> |
---|
2657 | <property |
---|
2658 | name="BiotinSignalNoiseRatio" |
---|
2659 | title="Signal noise ratio" |
---|
2660 | description="The ratio of spot intensity to the standard deviation of the local background of all spots in the microarray. Spots with a low Signal/Noise Ratio can be quickly identified for later reviews." |
---|
2661 | column="snr" |
---|
2662 | channel="1" |
---|
2663 | type="float" |
---|
2664 | /> |
---|
2665 | <property |
---|
2666 | name="BiotinConfidence" |
---|
2667 | title="Confidence" |
---|
2668 | description="Confidence." |
---|
2669 | column="confidence" |
---|
2670 | channel="1" |
---|
2671 | type="int" |
---|
2672 | /> |
---|
2673 | <property |
---|
2674 | name="IgnoreFilter" |
---|
2675 | title="Ignore filter" |
---|
2676 | description="Whether the feature quantitation is used in down-stream analysis (flagging feature as valid): 0 = feature invalid (null data); 1 = feature valid." |
---|
2677 | column="ignore_filter" |
---|
2678 | type="int" |
---|
2679 | /> |
---|
2680 | <property |
---|
2681 | name="BiotinRatio" |
---|
2682 | title="Ratio" |
---|
2683 | description="The portion of the overall spot fluorescence contributed by biotin channel (normalized so that biotin channel contribution equals 1)." |
---|
2684 | column="ratio" |
---|
2685 | channel="1" |
---|
2686 | type="int" |
---|
2687 | /> |
---|
2688 | <property |
---|
2689 | name="BiotinPercent" |
---|
2690 | title="Percent" |
---|
2691 | description="The portion of the overall spot fluorescence contributed by biotin channel (in percent)." |
---|
2692 | column="precent" |
---|
2693 | channel="1" |
---|
2694 | type="float" |
---|
2695 | /> |
---|
2696 | <property |
---|
2697 | name="BiotinDiameterFilter" |
---|
2698 | title="Diameter filter" |
---|
2699 | description="Biotin diameter filter." |
---|
2700 | column="diameter_filter" |
---|
2701 | type="int" |
---|
2702 | /> |
---|
2703 | <property |
---|
2704 | name="BiotinAreaFilter" |
---|
2705 | title="Area filter" |
---|
2706 | description="Biotin area filter." |
---|
2707 | column="area_filter" |
---|
2708 | type="int" |
---|
2709 | /> |
---|
2710 | <property |
---|
2711 | name="BiotinFootprintFilter" |
---|
2712 | title="Footprint filter" |
---|
2713 | description="Biotin footprint filter." |
---|
2714 | column="footprint_filter" |
---|
2715 | type="int" |
---|
2716 | /> |
---|
2717 | <property |
---|
2718 | name="BiotinCircularityFilter" |
---|
2719 | title="Circularity filter" |
---|
2720 | description="Biotin circularity filter." |
---|
2721 | column="circularity_filter" |
---|
2722 | type="int" |
---|
2723 | /> |
---|
2724 | <property |
---|
2725 | name="BiotinSpotUniformityFilter" |
---|
2726 | title="Spot uniformity filter" |
---|
2727 | description="Biotin spot uniformity filter." |
---|
2728 | column="uniformity_filter" |
---|
2729 | type="int" |
---|
2730 | /> |
---|
2731 | <property |
---|
2732 | name="BiotinBkgUniformityFilter" |
---|
2733 | title="Background uniformity filter" |
---|
2734 | description="Biotin background uniformity filter." |
---|
2735 | column="bg_uniformity_filter" |
---|
2736 | type="int" |
---|
2737 | /> |
---|
2738 | <property |
---|
2739 | name="BiotinSignalNoiseRatioFilter" |
---|
2740 | title="Signal noise ratio filter" |
---|
2741 | description="Biotin signal noise ratio filter." |
---|
2742 | column="snr_filter" |
---|
2743 | type="int" |
---|
2744 | /> |
---|
2745 | <property |
---|
2746 | name="BiotinReplicateFilter" |
---|
2747 | title="Replicate filter" |
---|
2748 | description="Biotin replicate filter." |
---|
2749 | column="replicate_filter" |
---|
2750 | type="int" |
---|
2751 | /> |
---|
2752 | <intensity-formula |
---|
2753 | name="meanfg" |
---|
2754 | title="Mean FG" |
---|
2755 | description="Mean foreground intensity" |
---|
2756 | > |
---|
2757 | <formula |
---|
2758 | channel="1" |
---|
2759 | expression="raw('intensity')" |
---|
2760 | /> |
---|
2761 | </intensity-formula> |
---|
2762 | <intensity-formula |
---|
2763 | name="mean" |
---|
2764 | title="Mean FG - Mean BG" |
---|
2765 | description="Subtract mean background from mean foreground" |
---|
2766 | > |
---|
2767 | <formula |
---|
2768 | channel="1" |
---|
2769 | expression="raw('BiotinIntensity') - raw('BiotinBackground')" |
---|
2770 | /> |
---|
2771 | </intensity-formula> |
---|
2772 | </raw-data-type> |
---|
2773 | |
---|
2774 | <raw-data-type |
---|
2775 | id="quantarraycy" |
---|
2776 | channels="2" |
---|
2777 | name="QuantArray Cy" |
---|
2778 | table="RawDataQuantArrayCy" |
---|
2779 | > |
---|
2780 | <property |
---|
2781 | name="Cy3Intensity" |
---|
2782 | title="Mean Cy3 intensity" |
---|
2783 | description="Mean pixel Cy3 foreground intensity in Channel Cy3." |
---|
2784 | column="cy3_mean" |
---|
2785 | channel="1" |
---|
2786 | type="float" |
---|
2787 | /> |
---|
2788 | <property |
---|
2789 | name="Cy3Background" |
---|
2790 | title="Mean Cy3 background" |
---|
2791 | description="Mean pixel Cy3 background intensity in Channel Cy3." |
---|
2792 | column="cy3_mean_bg" |
---|
2793 | channel="1" |
---|
2794 | type="float" |
---|
2795 | /> |
---|
2796 | <property |
---|
2797 | name="Cy3Diameter" |
---|
2798 | title="Cy3 spot diameter" |
---|
2799 | description="Average diameter of the spot (in microns). Used to determine whether the spot is close to the expected size or is perhaps an artifact on the slide." |
---|
2800 | column="cy3_diameter" |
---|
2801 | channel="1" |
---|
2802 | type="float" |
---|
2803 | /> |
---|
2804 | <property |
---|
2805 | name="Cy3Area" |
---|
2806 | title="Cy3 spot area" |
---|
2807 | description="The area of the spot given as the number of pixels (in sq. microns). Used to determine whether the spot is close to the expected size or is an artifact on the slide." |
---|
2808 | column="cy3_area" |
---|
2809 | channel="1" |
---|
2810 | type="int" |
---|
2811 | /> |
---|
2812 | <property |
---|
2813 | name="Cy3Footprint" |
---|
2814 | title="Cy3 footprint" |
---|
2815 | description="The distance between the expected position of a spot and its actual measured position (in microns)." |
---|
2816 | column="cy3_footprint" |
---|
2817 | channel="1" |
---|
2818 | type="float" |
---|
2819 | /> |
---|
2820 | <property |
---|
2821 | name="Cy3Circularity" |
---|
2822 | title="Cy3 circularity" |
---|
2823 | description="Reports the degree to which a spot is close to a perfect circle. This measurement is used to determine the print quality of the microarray." |
---|
2824 | column="cy3_circularity" |
---|
2825 | channel="1" |
---|
2826 | type="float" |
---|
2827 | /> |
---|
2828 | <property |
---|
2829 | name="Cy3SpotUniformity" |
---|
2830 | title="Cy3 spot uniformity" |
---|
2831 | description="The uniformity of the pixels used in the spot foreground intensity calculation.This helps in determining the quality of the spot and aids in selecting the correct quantification output method." |
---|
2832 | column="cy3_uniformity" |
---|
2833 | channel="1" |
---|
2834 | type="float" |
---|
2835 | /> |
---|
2836 | <property |
---|
2837 | name="Cy3BkgUniformity" |
---|
2838 | title="Cy3 background uniformity" |
---|
2839 | description="The uniformity of the pixels used in the background intensity calculation." |
---|
2840 | column="cy3_bg_uniformity" |
---|
2841 | channel="1" |
---|
2842 | type="float" |
---|
2843 | /> |
---|
2844 | <property |
---|
2845 | name="Cy3SignalNoiseRatio" |
---|
2846 | title="Cy3 signal noise ratio" |
---|
2847 | description="The ratio of spot intensity to the standard deviation of the local background of all spots in the microarray. Spots with a low Signal/Noise Ratio can be quickly identified for later reviews." |
---|
2848 | column="cy3_snr" |
---|
2849 | channel="1" |
---|
2850 | type="float" |
---|
2851 | /> |
---|
2852 | <property |
---|
2853 | name="Cy3Confidence" |
---|
2854 | title="Cy3 confidence" |
---|
2855 | description="Unused QuantArray feature, will always = 1." |
---|
2856 | column="cy3_confidence" |
---|
2857 | channel="2" |
---|
2858 | type="int" |
---|
2859 | /> |
---|
2860 | <property |
---|
2861 | name="Cy5Intensity" |
---|
2862 | title="Mean Cy5 intensity" |
---|
2863 | description="Mean pixel Cy5 foreground intensity in Channel Cy5." |
---|
2864 | column="cy5_mean" |
---|
2865 | channel="2" |
---|
2866 | type="float" |
---|
2867 | /> |
---|
2868 | <property |
---|
2869 | name="Cy5Background" |
---|
2870 | title="Mean Cy5 background" |
---|
2871 | description="Mean pixel Cy5 background intensity in Channel Cy5." |
---|
2872 | column="cy5_mean_bg" |
---|
2873 | channel="2" |
---|
2874 | type="float" |
---|
2875 | /> |
---|
2876 | <property |
---|
2877 | name="Cy5Diameter" |
---|
2878 | title="Cy5 spot diameter" |
---|
2879 | description="Average diameter of the spot (in microns). Used to determine whether the spot is close to the expected size or is perhaps an artifact on the slide." |
---|
2880 | column="cy5_diameter" |
---|
2881 | channel="2" |
---|
2882 | type="float" |
---|
2883 | /> |
---|
2884 | <property |
---|
2885 | name="Cy5Area" |
---|
2886 | title="Cy5 spot area" |
---|
2887 | description="The area of the spot given as the number of pixels (in sq. microns). Used to determine whether the spot is close to the expected size or is an artifact on the slide." |
---|
2888 | column="cy5_area" |
---|
2889 | channel="2" |
---|
2890 | type="int" |
---|
2891 | /> |
---|
2892 | <property |
---|
2893 | name="Cy5Footprint" |
---|
2894 | title="Cy5 footprint" |
---|
2895 | description="The distance between the expected position of a spot and its actual measured position (in microns)." |
---|
2896 | column="cy5_footprint" |
---|
2897 | channel="2" |
---|
2898 | type="float" |
---|
2899 | /> |
---|
2900 | <property |
---|
2901 | name="Cy5Circularity" |
---|
2902 | title="Cy5 circularity" |
---|
2903 | description="Reports the degree to which a spot is close to a perfect circle. This measurement is used to determine the print quality of the microarray." |
---|
2904 | column="cy5_circularity" |
---|
2905 | channel="2" |
---|
2906 | type="float" |
---|
2907 | /> |
---|
2908 | <property |
---|
2909 | name="Cy5SpotUniformity" |
---|
2910 | title="Cy5 spot uniformity" |
---|
2911 | description="The uniformity of the pixels used in the spot foreground intensity calculation.This helps in determining the quality of the spot and aids in selecting the correct quantification output method." |
---|
2912 | column="cy5_uniformity" |
---|
2913 | channel="2" |
---|
2914 | type="float" |
---|
2915 | /> |
---|
2916 | <property |
---|
2917 | name="Cy5BkgUniformity" |
---|
2918 | title="Cy5 background uniformity" |
---|
2919 | description="The uniformity of the pixels used in the background intensity calculation." |
---|
2920 | column="cy5_bg_uniformity" |
---|
2921 | channel="2" |
---|
2922 | type="float" |
---|
2923 | /> |
---|
2924 | <property |
---|
2925 | name="Cy5SignalNoiseRatio" |
---|
2926 | title="Cy5 signal noise ratio" |
---|
2927 | description="The ratio of spot intensity to the standard deviation of the local background of all spots in the microarray. Spots with a low Signal/Noise Ratio can be quickly identified for later reviews." |
---|
2928 | column="cy5_snr" |
---|
2929 | channel="2" |
---|
2930 | type="float" |
---|
2931 | /> |
---|
2932 | <property |
---|
2933 | name="Cy5Confidence" |
---|
2934 | title="Cy5 confidence" |
---|
2935 | description="Unused QuantArray feature, will always = 1." |
---|
2936 | column="cy5_confidence" |
---|
2937 | channel="2" |
---|
2938 | type="int" |
---|
2939 | /> |
---|
2940 | <property |
---|
2941 | name="IgnoreFilter" |
---|
2942 | title="Ignore filter" |
---|
2943 | description="Whether the feature quantitation is used in down-stream analysis (flagging feature as valid): 0 = feature invalid (null data); 1 = feature valid." |
---|
2944 | column="ignore_filter" |
---|
2945 | type="int" |
---|
2946 | /> |
---|
2947 | <property |
---|
2948 | name="Cy3Ratio" |
---|
2949 | title="Cy3 ratio" |
---|
2950 | description="The portion of the overall spot fluorescence contributed by channel Cy3 (normalized so that channel Cy3 contribution equals 1)." |
---|
2951 | column="cy3_ratio" |
---|
2952 | channel="1" |
---|
2953 | type="float" |
---|
2954 | /> |
---|
2955 | <property |
---|
2956 | name="Cy3Percent" |
---|
2957 | title="Cy3 precent" |
---|
2958 | description="The portion of the overall spot fluorescence contributed by channel Cy3 (in percent)." |
---|
2959 | column="cy3_percent" |
---|
2960 | channel="1" |
---|
2961 | type="float" |
---|
2962 | /> |
---|
2963 | <property |
---|
2964 | name="Cy3SignalNoiseRatioFilter" |
---|
2965 | title="Cy3 signal noise ratio filter" |
---|
2966 | description="Cy3 signal noise ratio filter" |
---|
2967 | column="cy3_snr_filter" |
---|
2968 | channel="1" |
---|
2969 | type="float" |
---|
2970 | /> |
---|
2971 | <property |
---|
2972 | name="Cy5Ratio" |
---|
2973 | title="Cy5 ratio" |
---|
2974 | description="The portion of the overall spot fluorescence contributed by channel Cy5 (normalized so that channel Cy5 contribution equals 1)." |
---|
2975 | column="cy5_ratio" |
---|
2976 | channel="2" |
---|
2977 | type="float" |
---|
2978 | /> |
---|
2979 | <property |
---|
2980 | name="Cy5Percent" |
---|
2981 | title="Cy5 precent" |
---|
2982 | description="The portion of the overall spot fluorescence contributed by channel Cy5 (in percent)." |
---|
2983 | column="cy5_percent" |
---|
2984 | channel="2" |
---|
2985 | type="float" |
---|
2986 | /> |
---|
2987 | <property |
---|
2988 | name="Cy5SignalNoiseRatioFilter" |
---|
2989 | title="Cy5 signal noise ratio filter" |
---|
2990 | description="Cy5 signal noise ratio filter" |
---|
2991 | column="cy5_snr_filter" |
---|
2992 | channel="2" |
---|
2993 | type="float" |
---|
2994 | /> |
---|
2995 | <property |
---|
2996 | name="log_normalised" |
---|
2997 | title="Normalized ratio" |
---|
2998 | description="Ratio = log2 Cy5 - log2 Cy3, for dyeswap ratio *-1" |
---|
2999 | column="log_normalised" |
---|
3000 | type="float" |
---|
3001 | /> |
---|
3002 | <intensity-formula |
---|
3003 | name="meanfg" |
---|
3004 | title="Mean FG" |
---|
3005 | description="Mean foreground intensity" |
---|
3006 | > |
---|
3007 | <formula |
---|
3008 | channel="1" |
---|
3009 | expression="raw('Cy3Intensity')" |
---|
3010 | /> |
---|
3011 | <formula |
---|
3012 | channel="2" |
---|
3013 | expression="raw('Cy5Intensity')" |
---|
3014 | /> |
---|
3015 | </intensity-formula> |
---|
3016 | <intensity-formula |
---|
3017 | name="mean" |
---|
3018 | title="Mean FG - Mean BG" |
---|
3019 | description="Subtract mean background from mean foreground" |
---|
3020 | > |
---|
3021 | <formula |
---|
3022 | channel="1" |
---|
3023 | expression="raw('Cy3Intensity') - raw('Cy3Background')" |
---|
3024 | /> |
---|
3025 | <formula |
---|
3026 | channel="2" |
---|
3027 | expression="raw('Cy5Intensity') - raw('Cy5Background')" |
---|
3028 | /> |
---|
3029 | </intensity-formula> |
---|
3030 | </raw-data-type> |
---|
3031 | |
---|
3032 | <raw-data-type |
---|
3033 | id="spotfinder" |
---|
3034 | channels="2" |
---|
3035 | name="Spotfinder" |
---|
3036 | table="RawDataSpotfinder" |
---|
3037 | > |
---|
3038 | <property |
---|
3039 | name="intA" |
---|
3040 | title="Intensity A" |
---|
3041 | description="Spot intensity in channel A corrected for background." |
---|
3042 | column="int_a" |
---|
3043 | type="int" |
---|
3044 | channel="1" |
---|
3045 | /> |
---|
3046 | <property |
---|
3047 | name="intB" |
---|
3048 | title="Intensity B" |
---|
3049 | description="Spot intensity in channel B corrected for background." |
---|
3050 | column="int_b" |
---|
3051 | type="int" |
---|
3052 | channel="2" |
---|
3053 | /> |
---|
3054 | <property |
---|
3055 | name="meanRatio" |
---|
3056 | title="Mean ratio" |
---|
3057 | description="Spot mean ratio." |
---|
3058 | column="mean_ratio" |
---|
3059 | type="float" |
---|
3060 | /> |
---|
3061 | <property |
---|
3062 | name="area" |
---|
3063 | title="Area" |
---|
3064 | description="Spot total area in pixels." |
---|
3065 | column="area" |
---|
3066 | type="int" |
---|
3067 | /> |
---|
3068 | <property |
---|
3069 | name="saturation" |
---|
3070 | title="Saturation factor" |
---|
3071 | description="Spot saturation factor. This measure shows the percentage of non-saturated pixels in the spot used for integration." |
---|
3072 | column="saturation" |
---|
3073 | type="float" |
---|
3074 | /> |
---|
3075 | <property |
---|
3076 | name="medianRatio" |
---|
3077 | title="Median ratio" |
---|
3078 | description="Spot median ratio." |
---|
3079 | column="median_ratio" |
---|
3080 | type="float" |
---|
3081 | /> |
---|
3082 | <property |
---|
3083 | name="modeRatio" |
---|
3084 | title="Mode ratio" |
---|
3085 | description="Spot mode ratio." |
---|
3086 | column="mode_ratio" |
---|
3087 | type="float" |
---|
3088 | /> |
---|
3089 | <property |
---|
3090 | name="bgA" |
---|
3091 | title="Background A" |
---|
3092 | description="Spot background in channel A." |
---|
3093 | column="bg_a" |
---|
3094 | channel="1" |
---|
3095 | type="int" |
---|
3096 | /> |
---|
3097 | <property |
---|
3098 | name="bgB" |
---|
3099 | title="Background B" |
---|
3100 | description="Spot background in channel B." |
---|
3101 | column="bg_b" |
---|
3102 | channel="2" |
---|
3103 | type="int" |
---|
3104 | /> |
---|
3105 | <property |
---|
3106 | name="flagA" |
---|
3107 | title="Flag A" |
---|
3108 | description="Spot flag in channel A. This flag is set by QC filter." |
---|
3109 | column="flag_a" |
---|
3110 | channel="1" |
---|
3111 | type="string" |
---|
3112 | /> |
---|
3113 | <property |
---|
3114 | name="flagB" |
---|
3115 | title="Flag B" |
---|
3116 | description="Spot flag in channel B. This flag is set by QC filter." |
---|
3117 | column="flag_b" |
---|
3118 | channel="2" |
---|
3119 | type="string" |
---|
3120 | /> |
---|
3121 | <property |
---|
3122 | name="qcA" |
---|
3123 | title="Spot QC score A" |
---|
3124 | description="Spot QC score in channel A. This is a geometric mean of shape and S/N QC scores in channel A. S/N QC score is calculated as percentage of pixels in a spot with values higher than 2*median(local BKG). Spot shape QC score is defined as ratio of spot area to spot perimeter scaled into the range between 0 and 1." |
---|
3125 | column="qc_a" |
---|
3126 | channel="1" |
---|
3127 | type="float" |
---|
3128 | /> |
---|
3129 | <property |
---|
3130 | name="qcB" |
---|
3131 | title="Spot QC score B" |
---|
3132 | description="Spot QC score in channel B. This is a geometric mean of shape and S/N QC scores in channel B. S/N QC score is calculated as percentage of pixels in a spot with values higher than 2*median(local BKG). Spot shape QC score is defined as ratio of spot area to spot perimeter scaled into the range between 0 and 1." |
---|
3133 | column="qc_b" |
---|
3134 | channel="2" |
---|
3135 | type="float" |
---|
3136 | /> |
---|
3137 | <property |
---|
3138 | name="qcTotal" |
---|
3139 | title="Total QC" |
---|
3140 | description="Spot total QC score. This is a mean of QC scores in channels A and B." |
---|
3141 | column="qc_total" |
---|
3142 | type="float" |
---|
3143 | /> |
---|
3144 | <property |
---|
3145 | name="flag" |
---|
3146 | title="Flag" |
---|
3147 | description="Flag values are generated based on next conditions: A - the number of non-saturated pixels in spot is 0; B- number of non-saturated pixels in spot is between 30 and 50; C- number of non saturated pixels in spot is more then 50; S- fully or partially saturated spot, this is a warning flag. User should evaluate used spot area. X- spot was detected and rejected based on spot shape and spot intensity relative to surrounding background; Z- spot was not detected by the program; Y- spot background is higher than spot intensity." |
---|
3148 | column="flag" |
---|
3149 | type="string" |
---|
3150 | /> |
---|
3151 | <intensity-formula |
---|
3152 | name="int" |
---|
3153 | title="Spot intensity" |
---|
3154 | description="Spot intensity corrected for background." |
---|
3155 | > |
---|
3156 | <formula |
---|
3157 | channel="1" |
---|
3158 | expression="raw('intA')" |
---|
3159 | /> |
---|
3160 | <formula |
---|
3161 | channel="2" |
---|
3162 | expression="raw('intB')" |
---|
3163 | /> |
---|
3164 | </intensity-formula> |
---|
3165 | </raw-data-type> |
---|
3166 | </raw-data-types> |
---|
3167 | |
---|