1 | <?xml version="1.0" encoding="ISO-8859-1" standalone="no"?> |
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2 | <?xml-stylesheet type="text/xsl" href="QT.xsl"?> |
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3 | <!DOCTYPE MAGE-ML PUBLIC "-//OMG//DTD MAGE-ML 1.0//EN" "http://schema.omg.org/lsr/gene_expression/1.0/MAGE-ML.dtd"> |
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4 | <MAGE-ML identifier="EBI:eleh:Spotfinder QT XML"> |
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5 | <QuantitationType_package> |
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6 | <QuantitationType_assnlist> |
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7 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:R" name="R" isBackground="false"> |
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8 | <Descriptions_assnlist> |
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9 | <Description text="Spot row number in slide."> |
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10 | </Description> |
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11 | </Descriptions_assnlist> |
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12 | <Scale_assn> |
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13 | <OntologyEntry category="Scale" value="unscaled"> |
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14 | </OntologyEntry> |
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15 | </Scale_assn> |
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16 | <DataType_assn> |
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17 | <OntologyEntry category="DataType" value="integer"> |
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18 | </OntologyEntry> |
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19 | </DataType_assn> |
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20 | </SpecializedQuantitationType> |
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21 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:C" name="C" isBackground="false"> |
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22 | <Descriptions_assnlist> |
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23 | <Description text="Spot column number in slide."> |
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24 | </Description> |
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25 | </Descriptions_assnlist> |
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26 | <Scale_assn> |
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27 | <OntologyEntry category="Scale" value="unscaled"> |
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28 | </OntologyEntry> |
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29 | </Scale_assn> |
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30 | <DataType_assn> |
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31 | <OntologyEntry category="DataType" value="integer"> |
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32 | </OntologyEntry> |
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33 | </DataType_assn> |
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34 | </SpecializedQuantitationType> |
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35 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:MR" name="MR" isBackground="false"> |
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36 | <Descriptions_assnlist> |
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37 | <Description text="Spot metarow number or subrow number."> |
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38 | </Description> |
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39 | </Descriptions_assnlist> |
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40 | <Scale_assn> |
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41 | <OntologyEntry category="Scale" value="unscaled"> |
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42 | </OntologyEntry> |
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43 | </Scale_assn> |
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44 | <DataType_assn> |
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45 | <OntologyEntry category="DataType" value="integer"> |
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46 | </OntologyEntry> |
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47 | </DataType_assn> |
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48 | </SpecializedQuantitationType> |
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49 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:MC" name="MC" isBackground="false"> |
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50 | <Descriptions_assnlist> |
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51 | <Description text="Spot metacolumn number or subcolumn number."> |
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52 | </Description> |
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53 | </Descriptions_assnlist> |
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54 | <Scale_assn> |
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55 | <OntologyEntry category="Scale" value="unscaled"> |
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56 | </OntologyEntry> |
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57 | </Scale_assn> |
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58 | <DataType_assn> |
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59 | <OntologyEntry category="DataType" value="integer"> |
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60 | </OntologyEntry> |
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61 | </DataType_assn> |
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62 | </SpecializedQuantitationType> |
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63 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:SR" name="SR" isBackground="false"> |
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64 | <Descriptions_assnlist> |
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65 | <Description text="Spot row number in a block/grid."> |
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66 | </Description> |
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67 | </Descriptions_assnlist> |
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68 | <Scale_assn> |
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69 | <OntologyEntry category="Scale" value="unscaled"> |
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70 | </OntologyEntry> |
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71 | </Scale_assn> |
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72 | <DataType_assn> |
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73 | <OntologyEntry category="DataType" value="integer"> |
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74 | </OntologyEntry> |
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75 | </DataType_assn> |
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76 | </SpecializedQuantitationType> |
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77 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:SC" name="SC" isBackground="false"> |
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78 | <Descriptions_assnlist> |
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79 | <Description text="Spot column number in a block/grid."> |
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80 | </Description> |
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81 | </Descriptions_assnlist> |
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82 | <Scale_assn> |
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83 | <OntologyEntry category="Scale" value="unscaled"> |
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84 | </OntologyEntry> |
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85 | </Scale_assn> |
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86 | <DataType_assn> |
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87 | <OntologyEntry category="DataType" value="integer"> |
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88 | </OntologyEntry> |
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89 | </DataType_assn> |
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90 | </SpecializedQuantitationType> |
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91 | <MeasuredSignal identifier="TIGR:Spotfinder_QuantitationType:IA" name="IA" isBackground="false"> |
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92 | <Descriptions_assnlist> |
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93 | <Description text="Spot intensity in channel A corrected for background."> |
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94 | </Description> |
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95 | </Descriptions_assnlist> |
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96 | <Channel_assnref> |
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97 | <Channel_ref identifier="ebi.ac.uk:Channel:A"/> |
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98 | </Channel_assnref> |
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99 | <Scale_assn> |
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100 | <OntologyEntry category="Scale" value="linear_scale"> |
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101 | </OntologyEntry> |
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102 | </Scale_assn> |
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103 | <DataType_assn> |
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104 | <OntologyEntry category="DataType" value="integer"> |
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105 | </OntologyEntry> |
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106 | </DataType_assn> |
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107 | </MeasuredSignal> |
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108 | <MeasuredSignal identifier="TIGR:Spotfinder_QuantitationType:IB" name="IB" isBackground="false"> |
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109 | <Descriptions_assnlist> |
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110 | <Description text="Spot intensity in channel B corrected for background."> |
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111 | </Description> |
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112 | </Descriptions_assnlist> |
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113 | <Channel_assnref> |
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114 | <Channel_ref identifier="ebi.ac.uk:Channel:B"/> |
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115 | </Channel_assnref> |
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116 | <Scale_assn> |
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117 | <OntologyEntry category="Scale" value="linear_scale"> |
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118 | </OntologyEntry> |
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119 | </Scale_assn> |
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120 | <DataType_assn> |
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121 | <OntologyEntry category="DataType" value="integer"> |
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122 | </OntologyEntry> |
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123 | </DataType_assn> |
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124 | </MeasuredSignal> |
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125 | <Ratio identifier="TIGR:Spotfinder_QuantitationType:MeanR" name="MeanR" isBackground="false"> |
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126 | <Descriptions_assnlist> |
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127 | <Description text="Spot mean ratio."> |
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128 | </Description> |
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129 | </Descriptions_assnlist> |
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130 | <Scale_assn> |
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131 | <OntologyEntry category="Scale" value="linear_scale"> |
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132 | </OntologyEntry> |
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133 | </Scale_assn> |
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134 | <DataType_assn> |
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135 | <OntologyEntry category="DataType" value="float"> |
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136 | </OntologyEntry> |
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137 | </DataType_assn> |
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138 | </Ratio> |
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139 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:SA" name="SA" isBackground="false"> |
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140 | <Descriptions_assnlist> |
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141 | <Description text="Spot total area in pixels."> |
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142 | </Description> |
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143 | </Descriptions_assnlist> |
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144 | <Scale_assn> |
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145 | <OntologyEntry category="Scale" value="linear_scale"> |
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146 | </OntologyEntry> |
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147 | </Scale_assn> |
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148 | <DataType_assn> |
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149 | <OntologyEntry category="DataType" value="integer"> |
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150 | </OntologyEntry> |
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151 | </DataType_assn> |
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152 | </SpecializedQuantitationType> |
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153 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:Sat" name="Sat" isBackground="false"> |
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154 | <Descriptions_assnlist> |
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155 | <Description text="Spot saturation factor. This measure shows the percentage of non-saturated pixels in the spot used for integration."> |
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156 | </Description> |
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157 | </Descriptions_assnlist> |
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158 | <Scale_assn> |
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159 | <OntologyEntry category="Scale" value="linear_scale"> |
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160 | </OntologyEntry> |
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161 | </Scale_assn> |
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162 | <DataType_assn> |
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163 | <OntologyEntry category="DataType" value="float"> |
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164 | </OntologyEntry> |
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165 | </DataType_assn> |
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166 | </SpecializedQuantitationType> |
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167 | <Ratio identifier="TIGR:Spotfinder_QuantitationType:MedianR" name="MedianR" isBackground="false"> |
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168 | <Descriptions_assnlist> |
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169 | <Description text="Spot median ratio."> |
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170 | </Description> |
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171 | </Descriptions_assnlist> |
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172 | <Scale_assn> |
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173 | <OntologyEntry category="Scale" value="linear_scale"> |
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174 | </OntologyEntry> |
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175 | </Scale_assn> |
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176 | <DataType_assn> |
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177 | <OntologyEntry category="DataType" value="float"> |
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178 | </OntologyEntry> |
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179 | </DataType_assn> |
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180 | </Ratio> |
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181 | <Ratio identifier="TIGR:Spotfinder_QuantitationType:ModeR" name="ModeR" isBackground="false"> |
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182 | <Descriptions_assnlist> |
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183 | <Description text="Spot mode ratio."> |
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184 | </Description> |
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185 | </Descriptions_assnlist> |
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186 | <Scale_assn> |
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187 | <OntologyEntry category="Scale" value="linear_scale"> |
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188 | </OntologyEntry> |
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189 | </Scale_assn> |
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190 | <DataType_assn> |
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191 | <OntologyEntry category="DataType" value="float"> |
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192 | </OntologyEntry> |
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193 | </DataType_assn> |
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194 | </Ratio> |
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195 | <MeasuredSignal identifier="TIGR:Spotfinder_QuantitationType:BGA" name="BGA" isBackground="true"> |
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196 | <Descriptions_assnlist> |
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197 | <Description text="Spot background in channel A."> |
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198 | </Description> |
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199 | </Descriptions_assnlist> |
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200 | <Channel_assnref> |
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201 | <Channel_ref identifier="ebi.ac.uk:Channel:A"/> |
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202 | </Channel_assnref> |
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203 | <Scale_assn> |
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204 | <OntologyEntry category="Scale" value="linear_scale"> |
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205 | </OntologyEntry> |
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206 | </Scale_assn> |
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207 | <DataType_assn> |
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208 | <OntologyEntry category="DataType" value="integer"> |
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209 | </OntologyEntry> |
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210 | </DataType_assn> |
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211 | </MeasuredSignal> |
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212 | <MeasuredSignal identifier="TIGR:Spotfinder_QuantitationType:BGB" name="BGB" isBackground="true"> |
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213 | <Descriptions_assnlist> |
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214 | <Description text="Spot background in channel B."> |
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215 | </Description> |
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216 | </Descriptions_assnlist> |
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217 | <Channel_assnref> |
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218 | <Channel_ref identifier="ebi.ac.uk:Channel:B"/> |
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219 | </Channel_assnref> |
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220 | <Scale_assn> |
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221 | <OntologyEntry category="Scale" value="linear_scale"> |
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222 | </OntologyEntry> |
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223 | </Scale_assn> |
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224 | <DataType_assn> |
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225 | <OntologyEntry category="DataType" value="integer"> |
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226 | </OntologyEntry> |
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227 | </DataType_assn> |
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228 | </MeasuredSignal> |
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229 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:FlagA" name="FlagA" isBackground="false"> |
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230 | <Descriptions_assnlist> |
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231 | <Description text="Spot flag in channel A. This flag is set by QC filter."> |
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232 | </Description> |
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233 | </Descriptions_assnlist> |
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234 | <Channel_assnref> |
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235 | <Channel_ref identifier="ebi.ac.uk:Channel:A"/> |
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236 | </Channel_assnref> |
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237 | <Scale_assn> |
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238 | <OntologyEntry category="Scale" value="unscaled"> |
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239 | </OntologyEntry> |
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240 | </Scale_assn> |
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241 | <DataType_assn> |
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242 | <OntologyEntry category="DataType" value="string_datatype"> |
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243 | </OntologyEntry> |
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244 | </DataType_assn> |
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245 | </SpecializedQuantitationType> |
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246 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:FlagB" name="FlagB" isBackground="false"> |
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247 | <Descriptions_assnlist> |
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248 | <Description text="Spot flag in channel B. This flag is set by QC filter."> |
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249 | </Description> |
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250 | </Descriptions_assnlist> |
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251 | <Channel_assnref> |
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252 | <Channel_ref identifier="ebi.ac.uk:Channel:B"/> |
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253 | </Channel_assnref> |
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254 | <Scale_assn> |
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255 | <OntologyEntry category="Scale" value="unscaled"> |
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256 | </OntologyEntry> |
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257 | </Scale_assn> |
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258 | <DataType_assn> |
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259 | <OntologyEntry category="DataType" value="string_datatype"> |
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260 | </OntologyEntry> |
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261 | </DataType_assn> |
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262 | </SpecializedQuantitationType> |
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263 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:QCA" name="QCA" isBackground="false"> |
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264 | <Descriptions_assnlist> |
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265 | <Description text="Spot QC score in channel A. This is a geometric mean of shape and S/N QC scores in channel A. S/N QC score is calculated as percentage of pixels in a spot with values higher than 2*median(local BKG). Spot shape QC score is defined as ratio of spot area to spot perimeter scaled into the range between 0 and 1."> |
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266 | </Description> |
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267 | </Descriptions_assnlist> |
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268 | <Channel_assnref> |
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269 | <Channel_ref identifier="ebi.ac.uk:Channel:A"/> |
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270 | </Channel_assnref> |
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271 | <Scale_assn> |
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272 | <OntologyEntry category="Scale" value="linear_scale"> |
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273 | </OntologyEntry> |
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274 | </Scale_assn> |
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275 | <DataType_assn> |
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276 | <OntologyEntry category="DataType" value="float"> |
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277 | </OntologyEntry> |
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278 | </DataType_assn> |
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279 | </SpecializedQuantitationType> |
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280 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:QCB" name="QCB" isBackground="false"> |
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281 | <Descriptions_assnlist> |
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282 | <Description text="Spot QC score in channel B. This is a geometric mean of shape and S/N QC scores in channel B. S/N QC score is calculated as percentage of pixels in a spot with values higher than 2*median(local BKG). Spot shape QC score is defined as ratio of spot area to spot perimeter scaled into the range between 0 and 1."> |
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283 | </Description> |
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284 | </Descriptions_assnlist> |
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285 | <Channel_assnref> |
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286 | <Channel_ref identifier="ebi.ac.uk:Channel:B"/> |
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287 | </Channel_assnref> |
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288 | <Scale_assn> |
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289 | <OntologyEntry category="Scale" value="linear_scale"> |
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290 | </OntologyEntry> |
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291 | </Scale_assn> |
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292 | <DataType_assn> |
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293 | <OntologyEntry category="DataType" value="float"> |
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294 | </OntologyEntry> |
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295 | </DataType_assn> |
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296 | </SpecializedQuantitationType> |
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297 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:QC" name="QC" isBackground="false"> |
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298 | <Descriptions_assnlist> |
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299 | <Description text="Spot total QC score. This is a mean of QC scores in channels A and B."> |
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300 | </Description> |
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301 | </Descriptions_assnlist> |
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302 | <Scale_assn> |
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303 | <OntologyEntry category="Scale" value="linear_scale"> |
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304 | </OntologyEntry> |
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305 | </Scale_assn> |
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306 | <DataType_assn> |
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307 | <OntologyEntry category="DataType" value="float"> |
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308 | </OntologyEntry> |
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309 | </DataType_assn> |
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310 | </SpecializedQuantitationType> |
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311 | <SpecializedQuantitationType identifier="TIGR:Spotfinder_QuantitationType:Flag" name="Flag" isBackground="false"> |
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312 | <Descriptions_assnlist> |
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313 | <Description text="Flag values are generated based on next conditions: A - the number of non-saturated pixels in spot is 0; B- number of non-saturated pixels in spot is between 30 and 50; C- number of non saturated pixels in spot is more then 50; S- fully or partially saturated spot, this is a warning flag. User should evaluate used spot area. X- spot was detected and rejected based on spot shape and spot intensity relative to surrounding background; Z- spot was not detected by the program; Y- spot background is higher than spot intensity."> |
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314 | </Description> |
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315 | </Descriptions_assnlist> |
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316 | <Scale_assn> |
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317 | <OntologyEntry category="Scale" value="unscaled"> |
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318 | </OntologyEntry> |
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319 | </Scale_assn> |
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320 | <DataType_assn> |
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321 | <OntologyEntry category="DataType" value="string_datatype"> |
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322 | </OntologyEntry> |
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323 | </DataType_assn> |
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324 | </SpecializedQuantitationType> |
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325 | </QuantitationType_assnlist> |
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326 | </QuantitationType_package> |
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327 | </MAGE-ML> |
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