Changeset 2954
- Timestamp:
- Nov 26, 2006, 9:38:24 PM (16 years ago)
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- 1 edited
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trunk/doc/user/getting_started.html
r2869 r2954 157 157 importer", and click on the 'New configuration ...' 158 158 tab. Enter a name in the 'Name' field (Affymetrix probe 159 set importer / ) and click 'Save and configure' 160 button. The next pop-up expects input of regular 161 expression needed to import reporter data. Here we just 162 give the values to add to the panel, but as of version 2.1 163 it is possible to use a import format wizard. The wizard 164 is started by clicking on the 'Test with file ...' 165 button. Here we tabulated the regexps to enter (assuming 166 that the default 159 set importer / Genepix reporter import) and click 'Save 160 and configure' button. The next pop-up expects input of 161 regular expression needed to import reporter data. Here we 162 just give the values to add to the panel, but as of 163 version 2.1 it is possible to use a import format 164 wizard. The wizard is started by clicking on the 'Test 165 with file ...' button. </p> 166 167 <p> Below we tabulate regexps to enter for a few file 168 formats if you prefer to enter the regexp manually. Note 169 1: Here it is assumed that the default 167 170 <a 168 171 href="../admin/extended-properties.html">extended-properties.xml</a> 169 is used): 170 <table> 171 <tr> 172 <td> <b>Column</b> </td> 173 <td> <b>Regexp</b> (Affymetrix) </td> 174 <td> <b>Regexp</b> (Genepix) </td> 172 is used. Note 2: your browser may break lines in the 173 table cells, treat these line breaks as a ' ' (space) 174 character. Note 3: Depending on your files the values to 175 input may be different. 176 <table cellspacing=3> 177 <tr> 178 <td align=left> <i>Column</i> </td> 179 <td align=left> <i>Affymetrix</i> </td> 180 <td align=left> <i>Genepix .gpr</i> </td> 181 <td align=left> <i>Genepix .txt</i> </td> 175 182 </tr> 176 183 <tr> 177 184 <td> Data Header </td> 178 185 <td> "Probe Set ID","GeneChip Array",.* </td> 179 <td> </td> 186 <td> "Block"\t"Column"\t"Row"\t"Name"\t"ID"\t.* </td> 187 <td> 384_number\t384_column\t384_row\t384_position\toligo_id.* </td> 180 188 </tr> 181 189 <tr> 182 190 <td> Data splitter </td> 183 191 <td> (?!"),(?=") </td> 184 <td> </td> 192 <td> \t</td> 193 <td> \t</td> 185 194 </tr> 186 195 <tr> 187 196 <td> Remove quotes </td> 188 197 <td> true </td> 189 <td> </td> 198 <td> true </td> 199 <td> true </td> 190 200 </tr> 191 201 <tr> 192 202 <td> Name </td> 193 203 <td> \Probe Set ID\ </td> 194 <td> </td> 204 <td> \Name\</td> 205 <td> \oligo_id\</td> 195 206 </tr> 196 207 <tr> 197 208 <td> Reporter ID </td> 198 209 <td> \Probe Set ID\ </td> 199 <td> </td> 210 <td> \ID\</td> 211 <td> \oligo_id\ </td> 200 212 </tr> 201 213 <tr> … … 203 215 <td> \Gene Symbol\ </td> 204 216 <td> </td> 217 <td> \gene_symbol_Ensembl*\ </td> 205 218 </tr> 206 219 <tr> … … 208 221 <td> \UniGene ID\ </td> 209 222 <td> </td> 223 <td> \RefSeq\ </td> 210 224 </tr> 211 225 </table> … … 216 230 do not need a configuration since these are not imported 217 231 to BASE with a plug-in. Genepix data requires a 218 configuration. Select the "Reporter map importer" from the 219 plug-in definition list. Genepix documentation missing 220 here. </p> 221 222 <p> <i>Raw data importer</i> <br> Affymetrix CEL files 223 do not need a configuration since these are not imported 224 to BASE with a plug-in. Genepix data requires a 225 configuration. Select the "Raw data importer" from the 226 plug-in definition list. Genepix documentation missing 227 here. </p> 232 configuration. To configure a plug-in for Genepix reporter 233 map imports select the "Reporter map importer" from the 234 plug-in definition list. Click on the 'New configuration 235 ...' tab. Enter a name in the 'Name' field (Affymetrix 236 probe set importer / Genepix reporter map importer) and 237 click 'Save and configure' button. The next pop-up expects 238 input of regular expression needed to import reporter map 239 data. Here we just give the values to add to the panel, 240 but as of version 2.1 it is possible to use a import 241 format wizard. The wizard is started by clicking on the 242 'Test with file ...' button. Below we tabulate regexps to 243 enter if you prefer to enter the regexp manually: 244 <table cellspacing=3> 245 <tr> 246 <td align=left> <i>Column</i> </td> 247 <td align=left> <i>Genepix .gpr</i> </td> 248 </tr> 249 <tr> 250 <td> Data Header </td> 251 <td> "Block"\t"Column"\t"Row"\t"Name"\t"ID"\t.* </td> 252 </tr> 253 <tr> 254 <td> Data splitter </td> 255 <td> \t</td> 256 </tr> 257 <tr> 258 <td> Remove quotes </td> 259 <td> true </td> 260 </tr> 261 <tr> 262 <td> Reporter ID </td> 263 <td> \ID\</td> 264 </tr> 265 <tr> 266 <td> Block </td> 267 <td> \Block\ </td> 268 </tr> 269 <tr> 270 <td> Column </td> 271 <td> \Column\ </td> 272 </tr> 273 <tr> 274 <td> Row </td> 275 <td> \Row\ </td> 276 </tr> 277 </table> 278 <br> Finalize with clicking 'Next'. You can of course fill 279 the other entries as well. </p> 280 281 <p> <i>Raw data importer</i> <br> Affymetrix CEL files do 282 not need a configuration since these are not imported to 283 BASE with a plug-in. Genepix data requires a 284 configuration. Create a configuration for Genepix raw data 285 import by selecting the "Raw data importer" from the 286 plug-in definition list. Click on the 'New configuration 287 ...' tab. Enter a name in the 'Name' field (Genepix raw 288 data importer) and click 'Save and configure' button. The 289 next pop-up expects input of regular expression needed to 290 import raw data. Here we just give the values to add to 291 the panel, but as of version 2.1 it is possible to use a 292 import format wizard. The wizard is started by clicking on 293 the 'Test with file ...' button. Below we tabulate 294 regexps to enter if you prefer to enter the regexp 295 manually: 296 297 <table cellspacing=3> 298 <tr> 299 <td align=left> <i>Column</i> </td> 300 <td align=left> <i>Genepix .gpr</i> </td> 301 </tr> 302 <tr> 303 <td>Raw data type</td> 304 <td>Genepix</td> 305 </tr> 306 <tr> 307 <td>Header</td> 308 <td>"(.+)=(.*)"</td> 309 </tr> 310 <tr> 311 <td>Data header</td> 312 <td>"Block"\t"Column"\t"Row"\t"Name"\t"ID"\t.*"Ratio of Medians \(532\/635\)".*</td> 313 </tr> 314 <tr> 315 <td>Data splitter</td> 316 <td>\t</td> 317 </tr> 318 <tr> 319 <td>Min data columns</td> 320 <td>48</td> 321 </tr> 322 <tr> 323 <td>Max data columns</td> 324 <td>48</td> 325 </tr> 326 <tr> 327 <td>Block</td> 328 <td>\Block\</td> 329 </tr> 330 <tr> 331 <td>Column</td> 332 <td>\Column\</td> 333 </tr> 334 <tr> 335 <td>Row</td> 336 <td>\Row\</td> 337 </tr> 338 <tr> 339 <td>X</td> 340 <td>\X\</td> 341 </tr> 342 <tr> 343 <td>Y</td> 344 <td>\Y\</td> 345 </tr> 346 <tr> 347 <td>Reporter ID</td> 348 <td>\ID\</td> 349 </tr> 350 <tr> 351 <td>Spot diameter</td> 352 <td>\Dia.\</td> 353 </tr> 354 <tr> 355 <td>Channel 1 foreground median</td> 356 <td>\F635 Median\</td> 357 </tr> 358 <tr> 359 <td>Channel 1 foreground mean</td> 360 <td>\F635 Mean\</td> 361 </tr> 362 <tr> 363 <td>Channel 1 foreground standard deviation</td> 364 <td>\F635 SD\</td> 365 </tr> 366 <tr> 367 <td>Channel 1 background median</td> 368 <td>\B635 Median\</td> 369 </tr> 370 <tr> 371 <td>Channel 1 background mean</td> 372 <td>\B635 Mean\</td> 373 </tr> 374 <tr> 375 <td>Channel 1 background standard deviation</td> 376 <td>\B635 SD\</td> 377 </tr> 378 <tr> 379 <td>Percent pixels within 1 standard deviation</td> 380 <td>\% > B635+1SD\</td> 381 </tr> 382 <tr> 383 <td>Percent pixels within 2 standard deviations</td> 384 <td>\% > B635+2SD\</td> 385 </tr> 386 <tr> 387 <td>Percent saturated pixels</td> 388 <td>\F635 % Sat.\</td> 389 </tr> 390 <tr> 391 <td>Channel 2 foreground median</td> 392 <td>\F532 Median\</td> 393 </tr> 394 <tr> 395 <td>Channel 2 foreground mean</td> 396 <td>\F532 Mean\</td> 397 </tr> 398 <tr> 399 <td>Channel 2 foreground standard deviation</td> 400 <td>\F532 SD\</td> 401 </tr> 402 <tr> 403 <td>Channel 2 background median</td> 404 <td>\B532 Median\</td> 405 </tr> 406 <tr> 407 <td>Channel 2 background mean</td> 408 <td>\B532 Mean\</td> 409 </tr> 410 <tr> 411 <td>Channel 2 background standard deviation</td> 412 <td>\B532 SD\</td> 413 </tr> 414 <tr> 415 <td>Percent pixels within 1 standard deviation</td> 416 <td>\% > B532+1SD\</td> 417 </tr> 418 <tr> 419 <td>Percent pixels within 2 standard deviations</td> 420 <td>\% > B532+2SD\</td> 421 </tr> 422 <tr> 423 <td>Percent saturated pixels</td> 424 <td>\F532 % Sat.\</td> 425 </tr> 426 <tr> 427 <td>Foreground pixels</td> 428 <td>\F Pixels\</td> 429 </tr> 430 <tr> 431 <td>Background pixels</td> 432 <td>\B Pixels\</td> 433 </tr> 434 <tr> 435 <td>Flags</td> 436 <td>\Flags\</td> 437 </tr> 438 </table> 439 <br> Finalize with clicking 'Next'. You can of course fill 440 the other entries as well. </p> 228 441 </li> 229 442 … … 284 497 <p> <i>Genepix reporter map import</i> <br> Click on the 285 498 newly created array design, and click on the 'Import...' 286 tab. More information needed here. </p> 499 tab. In the pop up, click 'Next'. Browse for the reporter 500 map file and click 'Next'. In the following dialogue you 501 can specify how to treat errors encountered. The default 502 is 'fail' which may be too restrictive, trial and error is 503 the way to go here for now. You can safely leave the 504 'Character set' as it is. </p> 287 505 </li> 288 506 … … 349 567 <li> 350 568 <p> <b>Analysing an experiment step I</b> <br> Select the 351 newly created experiment and in the the experiment window n569 newly created experiment and in the the experiment window 352 570 select the 'Bioassay sets' tab. There are no bioassay sets and 353 571 you must create a root bioassay set. This step is different … … 401 619 analysis tree.) So, assuming you select the proper export 402 620 button you will be given a list of different export 403 formats. The list of supported e ports depends on what plug-ins621 formats. The list of supported exports depends on what plug-ins 404 622 are added to the server, but there should at least be an 405 623 export to <a href=http://www.tm4.org/mev.html>MultiExperiment
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