Changeset 7640
- Timestamp:
- Mar 11, 2019, 2:13:29 PM (5 years ago)
- Location:
- trunk/doc/src
- Files:
-
- 1 deleted
- 9 edited
Legend:
- Unmodified
- Added
- Removed
-
trunk/doc/src/docbook/admin/installation.xml
r7631 r7640 93 93 by the upgrade script. The recommendation is to replace this feature with 94 94 an external files solution instead. 95 </para> 96 97 <bridgehead>Spot images support has been removed</bridgehead> 98 <para> 99 It is no longer possible to create new spot images or view existing 100 spot images via the BASE web client. Existing source image files and 101 zip archives with generated spot images have not been removed. 95 102 </para> 96 103 -
trunk/doc/src/docbook/appendix/coreplugins.xml
r6049 r7640 612 612 613 613 <varlistentry> 614 <term>Spot images creator</term>615 <listitem>616 <para>617 Converts a full-size image into JPEG images for each spot.618 No configuration is needed.619 </para>620 </listitem>621 </varlistentry>622 623 <varlistentry>624 614 <term>TAR file unpacker</term> 625 615 <listitem> -
trunk/doc/src/docbook/appendix/incompatible.xml
r7610 r7640 42 42 <para> 43 43 API that is related to the secondary storage feature has been removed. For example, 44 In the <classname docapi="net.sf.basedb.core">File</classname> class, <code>getAction()/setAction()</code>44 in the <classname docapi="net.sf.basedb.core">File</classname> class, <code>getAction()/setAction()</code> 45 45 has been removed. In the <classname docapi="net.sf.basedb.core">Location</classname> enumeration, 46 46 <code>SECONDARY</code> has been removed. 47 </para> 48 49 <bridgehead id="appendix.incompatible.spotimages">Spot images support has been removed</bridgehead> 50 <para> 51 API that is related to creating and viewing spot images has been removed. For example, 52 in the <classname docapi="net.sf.basedb.core">RawBioAssay</classname> class, the 53 <code>getSpotImages()</code> method has been removed. The <classname>SpotImages</classname> 54 class has been removed and there are several other minor changes related to this. 55 There is no replacement. 47 56 </para> 48 57 -
trunk/doc/src/docbook/appendix/update_warnings.xml
r7631 r7640 76 76 by the upgrade script. The recommendation is to replace this feature with 77 77 an external files solution instead. 78 </para> 79 80 <bridgehead>Spot images support has been removed</bridgehead> 81 <para> 82 It is no longer possible to create new spot images or view existing 83 spot images via the BASE web client. Existing source image files and 84 zip archives with generated spot images have not been removed. 78 85 </para> 79 86 -
trunk/doc/src/docbook/developer/base_api.xml
r7598 r7640 2233 2233 2234 2234 </sect3> 2235 2236 <sect3 id="data_api.rawdata.spotimages"> 2237 <title>Spot images</title> 2238 2239 <para> 2240 Spot images can be created if you have the original image 2241 files. BASE can use 1-3 images as sources for the red, green 2242 and blue channel respectively. The creation of spotimages requires 2243 that x and y coordinates are given for each raw data spot. The scaling 2244 and offset values are used to convert the coordinates to pixel 2245 coordinates. With this information BASE is able to cut out a square 2246 from the source images that, theoretically, contains a specific spot and 2247 nothing else. The spot images are gamma-corrected independently and then 2248 put together into PNG images that are stored in a zip file. 2249 </para> 2250 </sect3> 2251 2235 2252 2236 </sect2> 2253 2237 -
trunk/doc/src/docbook/user/project_permission.xml
r7222 r7640 89 89 <para> 90 90 Permission to add, update or delete annotations on an item. 91 In addition to this permission, < term>Use</term> permission91 In addition to this permission, <guilabel>Use</guilabel> permission 92 92 is required on the annotation type. 93 93 </para> -
trunk/doc/src/docbook/user/rawbioassays.xml
r5800 r7640 338 338 </sect2> 339 339 340 <sect2 id="experiments_analysis.spotimages">341 <title>Spot images</title>342 <para>343 This section only applies to microarray platforms where344 a coordinate system is used to identify spots on the345 array slides. The raw data must contain X and Y346 coordinates of each spot.347 </para>348 349 <para>350 After raw data has been imported into the database351 you will find that a <guibutton>Create spot images…</guibutton>352 button appears in the toolbar on the single-item view353 for the raw bioassay. Click on this button to open354 a window that allows you to specify parameters for the355 spot image extraction.356 </para>357 358 <figure id="experiments_analysis.figures.spotimage">359 <title>Create spot images</title>360 <screenshot>361 <mediaobject>362 <imageobject><imagedata363 fileref="figures/create_spot_images.png" format="PNG"364 /></imageobject>365 </mediaobject>366 </screenshot>367 </figure>368 369 <helptext external_id="rawbioassay.edit.spotimages"370 title="Create spot images">371 372 <variablelist>373 <varlistentry>374 <term><guilabel>X/Y scale and offset</guilabel></term>375 <listitem>376 <para>377 For the spot image creation process to be able378 to find the spots, the X and Y coordinates from379 the raw data must be converted into image pixel380 values. The formula used is:381 <code>pixelX = (rawX - offsetX) / scaleX</code>382 </para>383 384 <important>385 It is important that you get these values correct,386 or the spot image creation process may fail or generate387 incorrect spot images.388 </important>389 390 </listitem>391 </varlistentry>392 393 <varlistentry>394 <term><guilabel>Spot size</guilabel></term>395 <listitem>396 <para>397 The spot size is given in pixels and is the width and398 hight around each spot that is large enough to contain399 the spot without having too much empty space or400 neighbouring spots around it.401 </para>402 403 </listitem>404 </varlistentry>405 406 <varlistentry>407 <term><guilabel>Gamma correction</guilabel></term>408 <listitem>409 <para>410 Gamma correction is needed to make the images411 look good on computer displays. A value between412 1.8 and 2.2 is usually best.413 See <ulink url="http://en.wikipedia.org/wiki/Gamma_correction"414 >http://en.wikipedia.org/wiki/Gamma_correction</ulink>415 for more information.416 </para>417 418 </listitem>419 </varlistentry>420 421 <varlistentry>422 <term><guilabel>Quality</guilabel></term>423 <listitem>424 <para>425 The quality setting to use when saving the generated426 spot images as JPEG images. A value between 0 = poor427 and 1 = good can be used.428 </para>429 </listitem>430 </varlistentry>431 432 <varlistentry>433 <term><guilabel>Red, green and blue image files</guilabel></term>434 <listitem>435 <para>436 You must select which scanned image files to use for the437 red, green and blue component of the generated spot images.438 Use the <guibutton>Select…</guibutton> buttons439 to select existing images or upload new ones.440 The original image files must be 8- or441 16-bit grey scale images. Some scanners, for442 example Genepix, can create TIFF files with more than443 one image in each file. BASE supports this and uses444 the images in the order they appear in the TIFF file.445 </para>446 447 <note>448 Avoid TIFF images which also contains previews449 of the full image. BASE may use the wrong image450 with an error as the result. If you have multi-image451 TIFF files these must only contain the full images.452 </note>453 454 </listitem>455 </varlistentry>456 <varlistentry>457 <term><guilabel>Save as</guilabel></term>458 <listitem>459 <para>460 Specify the path and filename where the generated spot461 images should be saved. The process will create462 a single zip file containing all the images.463 </para>464 </listitem>465 </varlistentry>466 467 <varlistentry>468 <term><guilabel>Overwrite existing file</guilabel></term>469 <listitem>470 <para>471 If a file with the same name already exists you472 must mark this checkbox to overwrite it.473 </para>474 </listitem>475 </varlistentry>476 </variablelist>477 478 <para>479 Click on the <guibutton>Create</guibutton> button480 to add the spot image creation job to the job queue,481 or on &gbCancel; to abort.482 </para>483 484 </helptext>485 </sect2>486 340 </sect1> 487 341
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