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$Id: INSTALL 274 2007-05-21 17:00:16Z peter $
Copyright (C) 2006 Jari Häkkinen Copyright (C) 2007 Peter Johansson This file is part of se.lu.thep.affymetrix package for BASE, http://baseplugins.thep.lu.se/wiki/se.thep.lu.se.affymetrix se.lu.thep.affymetrix package for BASE is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. se.lu.thep.affymetrix package for BASE is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA.
Installation Guide for se.lu.thep.affymetrix
This file contains installation information for two plugins: RMAExpress and Plier.
If you think these instructions are worthless or stupid or maybe both, please tell me and I'll try to improve them. Even better, tell me how to improve them. My email address is jari@…
0. Introduction
Short intro:
The route for successful first time installation of this RMAExpress plugin is
- Install the plug-in.
- Install wxWidgets.
- Install RMAExpressConsole.
- Make BASE aware of the new plug-in.
The route for successful first time installation of this Plier plugin is
- Install the plug-in.
- Install apt-probeset-summarize.
- Make BASE aware of the new plug-in.
Upgrading the plug-in is even easier, see item 7) below.
Longer intro:
There are two supported ways to install this affymetrix package, you either download a pre-compiled package or you checkout the latest source from the subversion repository. The below instructions covers both installation options since major parts of the installation is the same for the different cases. There is also a source package available for download but installation from that package is currently not supported, it is supplied for reference only.
Note, the underlying algorithms RMA and Plier are not part of this package. This package contains the plug-in code needed for hooking up RMAExpress and Plier to BASE as a plug-in. The underlying binaries, RMAExpress and Plier, must be installed for the corresponding plug-in to work. See more in item 1.ii below.
Users who prefer the install from pre-compiled packages must go through all items below except 1.iii) and 2.iv).
Users who prefer the live on bleeding edge software and compiles the source from the subversion repository must go through all items except 3.v).
If you are upgrading a previously installed RMAExpress or Plier plug-in for BASE you can safely skip to item 7) below.
1. Pre-requisites
BASE 2
This plug-in package will only work with BASE 2.0 or later, i.e. BASE 1.2.x is not (and will never be) supported.
RMAExpressConsole and apt-probeset-summarize
Pre-requisite for RMAExpress plug-in (both binary package and subversion tree installs) is that RMAExpressConsole is installed.
Pre-requisite for Plier plug-in (both binary package and subversion tree installs) is that apt-probeset_summarize is installed.
RMAExpressConsole is available in the RMAExpress package written by Ben Bolstad, and the official RMAExpress web site is http://rmaexpress.bmbolstad.com/
You must download and compile RMAExpressConsole. This requires that wxWidgets (a.k.a. wxWindows) is installed on your system. wxWidgets is found at http://www.wxwidgets.org. The compilation of RMAExpressConsole is straightforward (at least on OpenSuSE 10.2 and MacOSX 10.4.8). Download v0.5alpha7, unpack and issue 'make'. This will compile RMAExpressConsole and som other binaries which are of no interest here.
apt-probeset-summarize is available in the Affymetrix Power Tools (APT) package available through Affymetrix home page http://www.affymetrix.com/support/developer/powertools/index.affx
APT is available as a Windows installer package and pre-built binaries for Linux and Mac OS-X. If you prefer building yourself, the source code is available as well. Though, it should be noted, we have experienced some problems building APT and at the time being we have not succeeded.
You should install the binaries (RMAExpressConsole and
apt-probeset-summarize) such that it is readable for the user running
your BASE application server. The name of the binaries MUST be
RMAExpressConsole
and apt-probeset-summarize
, respectively. Also
binaries must be copied into the same directory as the plug-in (see
item 3.v below). These two conditions are hard coded in the plug-ins.
Install of the BASE plug-in will succeed even if this requisite is not fulfilled, but execution of the plug-in within BASE will fail.
Compile time access to BASE core jar file.
This is only needed for compilation and installition from a subversion tree, i.e., it is not needed when installing a pre-compiled package.
The BASE2Core.jar (from BASE 2.0 and later) file in use by the server running the BASE web application must be available when compiling se.lu.thep.affymetrix.jar.
2. Compilation and installation from a subversion tree
You should skip this section if you downloaded a pre-compiled package.
iv) When installing from a subversion working copy you must compile the plug-in, do
make
This assumes that BASE is installed in /usr/local/base
. If this is not
the case do
make BASEROOT=<base-dir>
where <base-dir>
is to be replaced with the path to BASE. When the
compilation finishes you install the plug-in into the BASE application
server file system with
make install
ormake BASEROOT=<base-dir> install
The plug-in will install in the recommended directory
<base-dir>/www/plugins/se/lu/thep/affymetrix
, i.e., if you prefer
another location please refer to the Makefile (which supports further
options).
For advanced users only: <base-dir>
can really be any directory
readable for the user running your tomcat server. This may be handy if
you run several servers on one machine but only want one common
directory hierarchy for the plug-ins. Or, if you update the BASE
application frequently and in the process create new directories for
the newer application. More information about this is available in the
Makefile.
Remember the plug-in location for configuration of BASE below.
3. Installation from a pre-compiled package
You should skip this section if you use the subversion repository.
v) Install the plug-in in the BASE application server file system. If
BASE is installed in /usr/local/base
simply do
make install-precompiled
If BASE is installed elsewhere, do
make BASEROOT=<base-dir> install-precompiled
where <base-dir>
is to be replaced by the path to BASE. The plug-in
will install in the recommended directory
<base-dir>/www/plugins/se/lu/thep/affymetrix
, i.e., if you prefer
another location please refer to the Makefile (which supports further
options).
For advanced users only: <base-dir>
can really be any directory
readable for the user running your tomcat server. This may be handy if
you run several servers on one machine but only want one common
directory hierarchy for the plug-ins. Or, if you update the BASE
application frequently and in the process create new directories for
the newer application. More information about this is available in the
Makefile.
Remember the plug-in location for configuration of BASE below.
4. BASE configuration
vi) Make BASE aware of the new plug-in
- Login to BASE with plug-in administrative privileges.
- Choose 'Administrate' -> 'Plugins' -> 'Definitions'.
- Click on 'New...'. This will open up the 'Create plugin' pop-up window. Type "se.lu.thep.affymetrix.RMAExpress" in the 'Class' field and "<base-dir>/www/plugins/se/lu/thep/affymetrix/RMAExpress.jar" in the 'Path' field. Replace <base-dir> with whatever you used in item 3.v) above. Click on the 'Save' button.
- Share the plug-in to all users/roles/groups that should have access to it (sharing it to group Everyone should be sufficient in most cases).
- That's it, now you need to BASE skills to actually make use of the plug-in. Read the README file for some pointers on what to do now.
7. Updating a plug-in in BASE.
You may update the underlying binaries (RMAExpress or apt-probeset-summarize) or the plug-in.
Updating RMAExpressConsole or apt-probeset-summarize
Installing a new binary is trivial, just replace the old binary with the new binary.
Update RMAExpress or Plier plug-ins for BASE
This is straighforward:
- Replace the old jar-file and
- Login to BASE with plug-in administrative privileges.
- Choose 'Administrate' -> 'Plugins' -> 'Definitions'.
- Locate the plug-in (RMAExpress or PLier) in the list and click on it.
- Click on the 'Unload' button if it is available. This will remove the old plug-in from the application.
- Click on 'Edit...'. This will open up the 'Edit plugin' pop-up window. No parameter changes are need, just click on the 'Save' button.
- Done.