Changeset 1166 for plugins/base2
- Timestamp:
- Sep 28, 2009, 1:31:50 PM (14 years ago)
- Location:
- plugins/base2/net.sf.basedb.illumina/trunk
- Files:
-
- 2 edited
Legend:
- Unmodified
- Added
- Removed
-
plugins/base2/net.sf.basedb.illumina/trunk/INSTALL
r1142 r1166 1 1 ---------------------------------------------------------------------- 2 2 {{{ 3 Copyright (C) 2008 3 Copyright (C) 2008 Jari Häkkinen, Nicklas Nordborg 4 Copyright (C) 2009 Jari Häkkinen, Martin Svensson 4 5 5 6 This file is part of Illumina plug-in package for BASE. … … 68 69 15. Done. The Illumina plug-ins are now installed. You can now restart any job 69 70 agents as well. 71 16. Optionally, if not already done, disable use of the 'Illumina' 72 raw data type provided by a standard BASE installation; Simply 73 remove, or comment out, the `<raw-data-type>` entry for 74 `id='illumina'` in configuration file 75 `/path/to/base/WEB-INF/classes/raw-data-types.xml` and restart the 76 BASE server. If a user already used the 'Illumina' raw data type, 77 BASE will display an error message in the GUI. Either restore the 78 use of 'Illumina' raw data type or remove the data using 79 'Illumina' raw data type. 70 80 71 81 Make sure that the job agents have access to the illumina-plugins.jar file, … … 80 90 already installed Illumina package. 81 91 1. Do the step 1-3 as described in the installation instructions. 82 2. Then proceed with step 4. '''Caution:''' ''Any changes in the files on the local server must be transfer ed92 2. Then proceed with step 4. '''Caution:''' ''Any changes in the files on the local server must be transferred 83 93 to the new configuration-files before they are copied.'' Copying the new files will overwrite the existing files. 84 94 3. Do step 5-6 as described. 85 4. Step 7-9 are optional and only needed if the new release contains plugins or changed/new plugin configurations95 4. Step 7-9 are optional and only needed if the new release contains plugins or changed/new plugin configurations 86 96 that you want to install. [[BR]] 87 97 '''Notice1:''' Already installed plugins do not need to be re-installed(unless the jar-path has been changed), but updated configurations do.[[BR]] 88 98 '''Notice2:''' If the existing configuration is not first removed you will end up with two configurations with the same name. 89 '''Notice3:''' Upgrading from 1.3.x or less require you to run the installation wiz zard even for the installed plugins to update their jar-path.99 '''Notice3:''' Upgrading from 1.3.x or less require you to run the installation wizard even for the installed plugins to update their jar-path. 90 100 5. Step 10-13 are also optional and only necessary when the release comes with new Illumina variants that you want to create. 91 101 6. Do step 14 to update the extensions. -
plugins/base2/net.sf.basedb.illumina/trunk/README
r1097 r1166 1 1 ---------------------------------------------------------------------- 2 2 {{{ 3 Copyright (C) 2008 3 Copyright (C) 2008 Nicklas Nordborg 4 Copyright (C) 2009 Jari Häkkinen, Nicklas Nordborg, Martin Svensson 4 5 5 6 This file is part of Illumina plug-in package for BASE. … … 47 48 The Illumina BeadArray Reader is a scanner that can read arrays including Illumina 48 49 Sentrix BeadChips and Sentrix Array Matrices (SAMs). Operation of the BeadArray 49 Reader and image a quisition from Sentrix arrays is handled by the Illumina BeadScan50 Reader and image acquisition from Sentrix arrays is handled by the Illumina BeadScan 50 51 software. 51 52 … … 85 86 The column content in an IBS file is described below. 86 87 - ''' Illumicode ''': A code corresponding to the Array_Address_Id in the 87 Illumina Sentrix Array binary manifest (BGX) file. Note that the Illumi nacode88 Illumina Sentrix Array binary manifest (BGX) file. Note that the Illumicode 88 89 is a string (or integer) of varying length. The Array_Address_Id is a string with 89 a fixed leng ht of 10 characters that consists of an Illuminacode padded with zeros.90 a fixed length of 10 characters that consists of an Illumicode padded with zeros. 90 91 - ''' N ''': The total number of beads used to calculate Mean GRN and Dev GRN. 91 92 - ''' Mean GRN ''': The mean intensity. 92 93 - ''' Dev GRN ''': Standard deviation of the mean intensity. 93 94 94 IBS files may contain some rows with Illumicode sthat are not represented in the95 IBS files may contain some rows with Illumicode that are not represented in the 95 96 BGX files. 96 97 Our interpretation is that some probes that used to be annotated with a gene has 97 98 later been considered poor by Illumina (as we have only observed an increase 98 in the number of unmatched probes for later bgxrevisions). To avoid that the99 in the number of unmatched probes for later BGX revisions). To avoid that the 99 100 raw data importer fails cause of some probes, listed in the IBS-file, couldn't 100 101 be found in BASE - you have to set plug-in parameter 'Probe not found=skip', … … 116 117 BGX files are tab separated text files composed of 3 sections named Heading, 117 118 Probes, and Controls respectively. The first section is the Heading section. 118 It is prece eded by a row containing the text [Heading]. In the Heading section119 It is preceded by a row containing the text [Heading]. In the Heading section 119 120 some information is presented including the number of Probes and Controls 120 121 described in the BGX file. See below for an example of the Heading section. … … 127 128 Number of Controls 1426 128 129 }}} 129 Following the Heading section is the Probes section w ich is preceeded by a row130 Following the Heading section is the Probes section which is preceded by a row 130 131 containing the text [Probes]. The first row of the Probes section, i.e., the row 131 132 after [Probes] contain the header for the Probes section. Following the Probes 132 section is the Controls section w ich is preceeded by a row containing the text133 section is the Controls section which is preceded by a row containing the text 133 134 [Controls]. The first row of the Controls section, i.e., the row after [Controls] 134 135 contain the header for the Controls section. Note that the header row for the … … 194 195 == Getting started == 195 196 196 1. Install this package as described by the instructions in the INSTALL file. 197 2. Import reporter annotations. You will need one or more BGX files for this. 197 1. Install this package as described by the instructions in the 198 INSTALL file. 199 2. Optionally, if not already done, disable use of the 'Illumina' raw 200 data type provided by a standard BASE installation; Simply remove, 201 or comment out, the `<raw-data-type>` entry for `id='illumina'` in 202 configuration file 203 `/path/to/base/WEB-INF/classes/raw-data-types.xml` and restart the 204 BASE server. If a user already used the 'Illumina' raw data type, 205 BASE will display an error message in the GUI. Either restore the 206 use of 'Illumina' raw data type or remove the data using 207 'Illumina' raw data type. 208 3. Import reporter annotations. You will need one or more BGX files for this. 198 209 BGX files can be downloaded from http://www.switchtoi.com/annotationfiles.ilmn. 199 210 * Upload the BGX file(s) to BASE. … … 204 215 * Finish the job registration and wait for the plug-in to complete. 205 216 * Repeat this one time for each BGX file. 206 3. Create array designs. You will need one array design for each BGX file.217 4. Create array designs. You will need one array design for each BGX file. 207 218 * Go to the Array LIMS -> Array designs menu. 208 219 * Click on the New button. … … 218 229 * Finish the job registration and wait for the plug-in to complete. 219 230 * Repeat this for each BGX file. 220 4. Import raw data. You will need one or two IBS files.231 5. Import raw data. You will need one or two IBS files. 221 232 * Upload the IBS file(s) to BASE. 222 233 * Go to the View -> Raw bioassays menu. … … 232 243 * Finish the job registration and wait for the plug-in to complete. 233 244 * Repeat this for each set of raw data files. 234 5. Add your raw data sets to an experiment.245 6. Add your raw data sets to an experiment. 235 246 236 247 Tip! Steps 1-3 only needs to be done a single time for a BASE installation. If more than … … 239 250 240 251 Tip! The data import step in (4) above can be done for an entire experiment at a time. 241 242
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