Changes between Version 9 and Version 10 of LabelFree


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Timestamp:
Jan 14, 2014, 11:00:04 AM (9 years ago)
Author:
Fredrik Levander
Comment:

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  • LabelFree

    v9 v10  
    44
    55 1. Upload peak lists and import these to the hits report. MGF files can be used for identification only. mzML files can be used both for identification and quantification. It is possible to import MGF files in this step, and then use mzML files with the same name (apart from the suffix) for the feature detection (see below).
    6  1. Perform the identification searches using the peak list that were registered in the previous step and import the results to the hits report
    7  1. Create a combined hits report to obtain combined FDR values for the peptide identifications
     6 1. Perform the identification searches using the peak lists that were registered in the previous step and import the results to the hits report
     7 1. Create a combined hits report to obtain combined FDR values (q-values)for the peptide identifications
    88 1. Perform feature detection using msInspect or OpenMS on mzML or mzXML files. This can be done [wiki:FeatureDetection directly from Proteios SE], and then the features are imported directly - in this case skip the next step.
    99 1. Upload the .peptides.tsv files, and the mzML or mzXML files used for feature finding. In the case of mzML files, they can be used both for the peptide identification and the quantification. Check all the .peptides.tsv files and extensions->import file[s]->msInspect feature importer.