source: trunk/test/bam.cc @ 3028

Last change on this file since 3028 was 3028, checked in by Peter, 10 years ago

functions to access, append, and remove aux field. refs #746

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File size: 4.3 KB
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1// $Id: bam.cc 3028 2013-04-21 00:37:31Z peter $
2//
3// Copyright (C) 2013 Peter Johansson
4//
5// This program is free software; you can redistribute it and/or modify
6// it under the terms of the GNU General Public License as published by
7// the Free Software Foundation; either version 3 of the License, or
8// (at your option) any later version.
9//
10// This program is distributed in the hope that it will be useful, but
11// WITHOUT ANY WARRANTY; without even the implied warranty of
12// MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the GNU
13// General Public License for more details.
14//
15// You should have received a copy of the GNU General Public License
16// along with this program. If not, see <http://www.gnu.org/licenses/>.
17
18#include <config.h>
19
20#include "Suite.h"
21
22#ifdef HAVE_LIBBAM
23#include "yat/omic/BamFile.h"
24#include "yat/omic/BamRead.h"
25#endif
26
27#include <cassert>
28#include <string>
29
30using namespace theplu::yat;
31
32void test1(test::Suite& suite);
33
34int main(int argc, char* argv[])
35{
36  test::Suite suite(argc, argv);
37#ifndef HAVE_LIBBAM
38  suite.out() << "no libbam\n";
39  return EXIT_SKIP;
40#endif
41#ifndef HAVE_SAMTOOLS
42  suite.out() << "no samtools\n";
43  return EXIT_SKIP;
44#endif
45#ifdef HAVE_LIBBAM
46  test1(suite);
47#endif
48  return suite.return_value();
49}
50
51#ifdef HAVE_LIBBAM
52using namespace omic;
53
54void test_aux(test::Suite& suite, const BamRead& b)
55{
56  suite.out() << "test aux\n";
57  BamRead bam(b);
58
59  char c = 'h';
60  int size = bam.aux_size();
61  bam.aux_append("ZZ", 'A', 1, (uint8_t*)(&c));
62  suite.out() << "size: " << size << " " << bam.aux_size() << "\n";
63  suite.add(bam.aux_size() == size+4);
64  bam.aux("ZZ");
65  char c1 = bam_aux2A(bam.aux("ZZ"));
66  suite.out() << c << "==" << c1 << "\n";
67  suite.add(c==c1);
68  bam.aux_del("ZZ");
69  if (!suite.add(bam.aux_size() == size))
70    suite.err() << "error: incorrect size: " << bam.aux_size() << "\n";
71}
72
73
74void test_cigar(test::Suite& suite, const BamRead& b, const BamHeader& hdr)
75{
76  BamRead bam(b);
77
78  suite.out() << "test cigar:\n";
79  suite.out() << bam.cigar_str() << "\n";
80  OutBamFile os("cigar_test.bam", hdr);
81  std::vector<uint32_t> cig;
82
83  bam.cigar(cig);
84  suite.out() << bam.cigar_str() << "\n";
85  suite.add(bam.cigar_str()=="");
86  os.write(bam);
87
88  cig.resize(1);
89  cig[0] = bam_cigar_gen(bam.sequence_length(), BAM_CMATCH);
90  bam.cigar(cig);
91  suite.out() << bam.cigar_str() << "\n";
92  suite.add(bam.cigar_str()=="100M");
93  os.write(bam);
94
95  cig.resize(3);
96  cig[0] = bam_cigar_gen(50, BAM_CMATCH);
97  cig[1] = bam_cigar_gen(2, BAM_CDEL);
98  cig[2] = bam_cigar_gen(50, BAM_CMATCH);
99  bam.cigar(cig);
100  suite.out() << bam.cigar_str() << "\n";
101  suite.add(bam.cigar_str()=="50M2D50M");
102  os.write(bam);
103
104  // check that other elements in data* is preserved
105  if (!suite.add(same_query_name(b, bam)))
106    suite.err() << "error: \n'" << b.name() << "'\n'" << bam.name() << "'\n";
107
108  if (!suite.add(b.sequence()==bam.sequence()))
109    suite.err() << "error: \n'" << b.sequence() << "'\n'"
110                << bam.sequence() << "'\n";
111
112  for (int32_t i=0; i<b.core().l_qseq; ++i)
113    if (bam.qual(i) != b.qual(i)) {
114      suite.err() << "error: qual: " << i << " " << bam.qual(i)
115                  << " not euqal " << b.qual(i) << "\n";
116      suite.add(false);
117    }
118
119  os.close();
120}
121
122void test1(test::Suite& suite)
123{
124  std::string file = "../../data/foo.sorted.bam";
125
126  InBamFile in;
127  in.open(file);
128  BamRead bam;
129  in.read(bam);
130  std::string str = "AGCTTTGTTATTATTTGAGGGTGTGGTTCAGTTGTAAACAGTGTATG"
131    "TTTTAGAATTTGTGTTATTGTGATGGCGATGACCAAGTACAACATATTTCCCA";
132  std::string seq = bam.sequence();
133  if (str!=seq) {
134    suite.err() << "incorrect sequence: '" << bam.sequence() << "'\n";
135    suite.err() << "expected:           '" << str << "'\n";
136    suite.add(false);
137  }
138  uint8_t c = bam.sequence(24);
139  if (c != 4) {
140    suite.add(false);
141    suite.err() << "error: sequence: " << static_cast<int>(c)
142                << " expected " << 4 << "\n";
143  }
144  bam.sequence(24, 8);
145  c = bam.sequence(24);
146  if (c != 8) {
147    suite.add(false);
148    suite.err() << "error: sequence: " << static_cast<int>(c)
149                << " expected " << 8 << "\n";
150  }
151  uint8_t q = bam.qual(24);
152  if (q != 72) {
153    suite.add(false);
154    suite.err() << "error: quality: " << static_cast<int>(q)
155                << " expected " << 72 << "\n";
156  }
157  uint8_t new_q = 60;
158  bam.qual(24, new_q);
159  q = bam.qual(24);
160  if (q != new_q) {
161    suite.add(false);
162    suite.err() << "error: quality: " << static_cast<int>(q)
163                << " expected " << new_q << "\n";
164  }
165  test_cigar(suite, bam, in.header());
166  test_aux(suite, bam);
167}
168#endif
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